Difference between revisions of "Part:BBa K804012:Experience"
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| + | ===TrpA Knockout Complementation by British Columbia iGEM 2012=== | ||
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| + | This part consists of an arabinose promoter, a strong RBS, and the TrpA coding gene. It is unable to complement a TrpA knockout, and the growth rate appears to unaffected by the amount of arabinose that is added. The data for the Rhamnose Inducible MetA Coding Gene is also shown on this graph. | ||
| + | https://static.igem.org/mediawiki/parts/thumb/d/d2/Rhamnose_part_test.png/800px-Rhamnose_part_test.png | ||
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| + | To generate this graph we cultured an E. coli TrpA knockout transformed with this part in M9 minimal media with the indicated concentrations of arabinose, and measured the OD600 every 15 minutes. | ||
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| + | - [[User:jacobtoth|Jacob Toth]] | ||
Revision as of 03:50, 4 October 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K804012
User Reviews
UNIQ366cbda5b32163f1-partinfo-00000000-QINU UNIQ366cbda5b32163f1-partinfo-00000001-QINU
TrpA Knockout Complementation by British Columbia iGEM 2012
This part consists of an arabinose promoter, a strong RBS, and the TrpA coding gene. It is unable to complement a TrpA knockout, and the growth rate appears to unaffected by the amount of arabinose that is added. The data for the Rhamnose Inducible MetA Coding Gene is also shown on this graph.
To generate this graph we cultured an E. coli TrpA knockout transformed with this part in M9 minimal media with the indicated concentrations of arabinose, and measured the OD600 every 15 minutes.