Difference between revisions of "Part:BBa K824012:Experience"
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| − | We grew liquid cultures for 24 hours with varying concentrations of | + | We grew liquid cultures for 24 hours with varying concentrations of Lead Acetate in the presence of our cells. After growth, the tests were spun down, then resuspended in 1X PBS (Phosphate Buffer Solution). The results were measured in fluorescence per OD 600. As shown with the graph below, our results were inconsistent. We did not incorporate a lead binding protein into our design. Also, we noticed a precipitate starting at the .5 mM concentration. We feel that the precipitate or the lead's toxicity interfered with our results. |
<center>https://static.igem.org/mediawiki/2012/c/c4/Leadgraph.jpg</center> | <center>https://static.igem.org/mediawiki/2012/c/c4/Leadgraph.jpg</center> | ||
Latest revision as of 23:06, 13 October 2012
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We grew liquid cultures for 24 hours with varying concentrations of Lead Acetate in the presence of our cells. After growth, the tests were spun down, then resuspended in 1X PBS (Phosphate Buffer Solution). The results were measured in fluorescence per OD 600. As shown with the graph below, our results were inconsistent. We did not incorporate a lead binding protein into our design. Also, we noticed a precipitate starting at the .5 mM concentration. We feel that the precipitate or the lead's toxicity interfered with our results.
