Difference between revisions of "Part:BBa K902019:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K902019 short</partinfo>
 
<partinfo>BBa_K902019 short</partinfo>
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===Design Notes===
 
===Design Notes===
−
It had to be mutated
+
The S7 nuclease was synthesized from IDT however there was a point mutation in the sequence that hindered the activity of the nuclease. Instead of the 'A' there was a 'T'. We did mutagenesis to change the mutation back to its original nucleotide. This brick has the S7 that has undergone mutagenesis and is functional.
−
 
+
  
 +
GTTTCTTCGA ATTCGCGGCC GCTTCTAGAT GACAGAGTAT CTCCTAAGCG CGGGCATCTG
 +
TATGGCCATT GTTTCGATCT TATTGATCGG CATGGCGATA TCGAACGTAT CTAAGGGCCA
 +
GTACGCGAAA CGCTTCTTCT TTTTCGCCAC GAGTTGCCTT GTCTTAACAT TAGTTGTTGT
 +
TTCTTCACTG TCGTCCAGTG CGAACGCCTC TCAAACCGAT AACGGTGTAA ATCGCAGCGG
 +
GAGCGAAGAT CCTACCGTGT ATTCCGCGAC CTCAACGAAA AAACTTCATA AGGAGCCCGC
 +
TACGCTCATC A*A*AGCCATTG ACGGAGACAC TGTAAAGCTG ATGTACAAAG GCCAACCGAT
 +
GACCTTTCGC TTGCTGCTGG TCGACACCCC AGAGACGAAA CATCCGAAAA AGGGCGTCGA
 +
GAAATACGGC CCGGAAGCAA GTGCGTTTAC CAAGAAGATG GTGGAAAATG CGAACAAAAT
 +
TGAAGTCGAA TTTGATAAAG GGCAGCGTAC CGACAAATAT GGTCGTGGAC TGGCCTACAT
 +
TTATGCTGAT GGTAAAATGG TGAATGAAGC ACTGGTGCGG CAGGGTCTGG CCAAAGTGGC
 +
TTATGTGTAT AAACCGAATA ATACTCACGA ACAGCTGCTG CGTAAATCCG AAGCACAGGC
 +
AAAAAAAGAA AAACTGAACA TTTGGAGCGA AGATAACGCG GATAGCGGTC AATAATAATA
 +
ATACTAGTAG CGGCCGCTGC AGGAAGAAAC
  
 
===Source===
 
===Source===

Revision as of 02:58, 4 October 2012

S7 micrococcal nuclease


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The S7 nuclease was synthesized from IDT however there was a point mutation in the sequence that hindered the activity of the nuclease. Instead of the 'A' there was a 'T'. We did mutagenesis to change the mutation back to its original nucleotide. This brick has the S7 that has undergone mutagenesis and is functional.

GTTTCTTCGA ATTCGCGGCC GCTTCTAGAT GACAGAGTAT CTCCTAAGCG CGGGCATCTG TATGGCCATT GTTTCGATCT TATTGATCGG CATGGCGATA TCGAACGTAT CTAAGGGCCA GTACGCGAAA CGCTTCTTCT TTTTCGCCAC GAGTTGCCTT GTCTTAACAT TAGTTGTTGT TTCTTCACTG TCGTCCAGTG CGAACGCCTC TCAAACCGAT AACGGTGTAA ATCGCAGCGG GAGCGAAGAT CCTACCGTGT ATTCCGCGAC CTCAACGAAA AAACTTCATA AGGAGCCCGC TACGCTCATC A*A*AGCCATTG ACGGAGACAC TGTAAAGCTG ATGTACAAAG GCCAACCGAT GACCTTTCGC TTGCTGCTGG TCGACACCCC AGAGACGAAA CATCCGAAAA AGGGCGTCGA GAAATACGGC CCGGAAGCAA GTGCGTTTAC CAAGAAGATG GTGGAAAATG CGAACAAAAT TGAAGTCGAA TTTGATAAAG GGCAGCGTAC CGACAAATAT GGTCGTGGAC TGGCCTACAT TTATGCTGAT GGTAAAATGG TGAATGAAGC ACTGGTGCGG CAGGGTCTGG CCAAAGTGGC TTATGTGTAT AAACCGAATA ATACTCACGA ACAGCTGCTG CGTAAATCCG AAGCACAGGC AAAAAAAGAA AAACTGAACA TTTGGAGCGA AGATAACGCG GATAGCGGTC AATAATAATA ATACTAGTAG CGGCCGCTGC AGGAAGAAAC

Source

This part was synthesized from IDT and is native to Staphylococcus aureus

References