Difference between revisions of "Part:BBa K833013"
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<partinfo>BBa_K833013 short</partinfo> | <partinfo>BBa_K833013 short</partinfo> | ||
| − | This part is a lactate sensor derived from site directed mutagenesis of the E. coli AraF protein. | + | This part is a lactate sensor derived from site directed mutagenesis of the E. coli AraF protein. This part is a periplasmic lactate sensor derived from site directed mutagenesis of the E. coli AraF protein to create the lactate sensor described by Looger et al. (Looger, LL. et al., Computational design of receptor and sensor proteins with novel functions (Nature 2003). TDue to the multiple mutations, two rounds of mutagenesis were necessary to incorporate all mutations, and several colonies had to be sequenced after each round in order to identify colonies which contained all of the mutations. |
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Latest revision as of 03:59, 4 October 2012
Lactate sensor derived from the AraF protein of E. coli
This part is a lactate sensor derived from site directed mutagenesis of the E. coli AraF protein. This part is a periplasmic lactate sensor derived from site directed mutagenesis of the E. coli AraF protein to create the lactate sensor described by Looger et al. (Looger, LL. et al., Computational design of receptor and sensor proteins with novel functions (Nature 2003). TDue to the multiple mutations, two rounds of mutagenesis were necessary to incorporate all mutations, and several colonies had to be sequenced after each round in order to identify colonies which contained all of the mutations.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 106