Difference between revisions of "Part:BBa K887004"

Revision as of 09:54, 2 October 2012

Plac+alsS+ilvC+ilvD(each preceded by own zinc-finger and RBS)+Ptet+B0032+kivD+B0015

This part contain four enzymes. Each of the former three enzymes(alsS, ilvC& ilvD) linked with a zinc finger. The three enzymes could convert pyruvate into 2-ketoisoalerate(the Intermediate of isobutanol). The final enzyme(kivD)could change 2-ketoisoalerate into isobutyraldehyde, which would be converted into isobutanol by ADH in E.coli. This is a complete circuit to produce isobutanol.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 7718
Illegal XhoI site found at 6049
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 6938
Illegal AgeI site found at 3158
Illegal AgeI site found at 4143
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 2744
Illegal BsaI site found at 5835
Illegal BsaI site found at 6092
Illegal BsaI.rc site found at 836
Illegal BsaI.rc site found at 1430
Illegal BsaI.rc site found at 3563

Revision as of 09:46, 2 October 2012 (view source) Max07 (Talk \| contribs) ← Older edit		Revision as of 09:54, 2 October 2012 (view source) Max07 (Talk \| contribs) Newer edit →
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	This part contain four enzymes. Each of the former three enzymes(alsS, ilvC& ilvD) linked with a zinc finger. The three enzymes could convert pyruvate into 2-ketoisoalerate(the Intermediate of isobutanol). The final enzyme(kivD)could change 2-ketoisoalerate into isobutyraldehyde, which would be converted into isobutanol by ADH in E.coli. This is a complete circuit to produce isobutanol.		This part contain four enzymes. Each of the former three enzymes(alsS, ilvC& ilvD) linked with a zinc finger. The three enzymes could convert pyruvate into 2-ketoisoalerate(the Intermediate of isobutanol). The final enzyme(kivD)could change 2-ketoisoalerate into isobutyraldehyde, which would be converted into isobutanol by ADH in E.coli. This is a complete circuit to produce isobutanol.