Difference between revisions of "Part:BBa K749015:Experience"
 
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The mixture was incubated up to 30 mitutes and formate was quantified by liquid chromatography.
 
The mixture was incubated up to 30 mitutes and formate was quantified by liquid chromatography.
  
−
The details and result are described in our wiki. →http://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay_3_Protocol_and_Result
+
The details and result are described in our wiki.
 +
 
 +
<p class="description3">
 +
only formate 2mM<Br>
 +
Peak = 8.81(min)<Br>
 +
<Br>
 +
<BR>
 +
<b>BBa_K749015</b>
 +
<Br>
 +
<table border>
 +
<tr>
 +
<td>Reaction time </td>
 +
<td> volume </td>
 +
<td> Peak area </td>
 +
<td> Peak area(%) </td>
 +
</tr>
 +
<tr>
 +
<td>30min </td>
 +
<td>50μl</td>
 +
<td>229371.2</td>
 +
<td>80.88</td>
 +
</tr>
 +
<tr>
 +
<td>10min</td>
 +
<td>20μl</td>
 +
<td>248294.2</td>
 +
<td>83.9823</td>
 +
</tr>
 +
</table>
 +
<Br>
 +
<b>WT</b><Br>
 +
<table border>
 +
<tr>
 +
<td>Reaction time </td>
 +
<td> volume </td>
 +
<td> Peak area </td>
 +
<td> Peak area(%) </td>
 +
</tr>
 +
<tr>
 +
<td>10min </td>
 +
<td>20μl</td>
 +
<td>232791.6</td>
 +
<td>83.691</td>
 +
</tr>
 +
</table>　
 +
<Br>
 +
 
 +
<b>No enzym</b><Br>
 +
<table border>
 +
<tr>
 +
<td>Reaction time </td>
 +
<td> volume </td>
 +
<td> Peak area </td>
 +
<td> Peak area(%) </td>
 +
</tr>
 +
<tr>
 +
<td>10min </td>
 +
<td>20μl</td>
 +
<td>243418.6</td>
 +
<td>84.37</td>
 +
</tr>
 +
</table>　
 +
 
 +
 
 +
 
 +
 
 +
→http://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay_3_Protocol_and_Result
  
 
===Applications of BBa_K749015===
 
===Applications of BBa_K749015===

Latest revision as of 07:16, 6 October 2012

E. coli transformated BBa_K749021 were cultured in LB medium overnight, disrupted by sonicator, and then extracted as the crude enzyme solution.
We mixed the emzyme solution 0.05 mL, 20 mM sodium formate 0.1 mL (2 mM final concentration), 20 mM NAD + 0.1 mL with 50 mM Tris-HCl pH 7.2 0.8 mL.
The mixture was incubated up to 30 mitutes and formate was quantified by liquid chromatography.

The details and result are described in our wiki.

only formate 2mM
Peak = 8.81(min)


BBa_K749015

Reaction time volume Peak area Peak area(%)
30min 50μl 229371.2 80.88
10min 20μl 248294.2 83.9823


WT

Reaction time volume Peak area Peak area(%)
10min 20μl 232791.6 83.691
　


No enzym

Reaction time volume Peak area Peak area(%)
10min 20μl 243418.6 84.37
　



→http://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay_3_Protocol_and_Result

Applications of BBa_K749015

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