Difference between revisions of "Part:BBa K749015"
 
Line 10: Line 10:
 
FDH4 is composed of α-subunit and β-subunit.
 
FDH4 is composed of α-subunit and β-subunit.
 
This parts consists both of them.<Br>
 
This parts consists both of them.<Br>
 +
 +
 +
Decompose quontify
 +
 +
only 2mM formate in medium<Br>
 +
Peak = 8.81(min)<Br>
 +
<Br>
 +
<BR>
 +
<b>BBa_K749015</b>
 +
<Br>
 +
<table border>
 +
<tr>
 +
<td>Reaction time </td>
 +
<td> volume </td>
 +
<td> Peak area </td>
 +
<td> Peak area(%) </td>
 +
</tr>
 +
<tr>
 +
<td>30min </td>
 +
<td>50μl</td>
 +
<td>229371.2</td>
 +
<td>80.88</td>
 +
</tr>
 +
<tr>
 +
<td>10min</td>
 +
<td>20μl</td>
 +
<td>248294.2</td>
 +
<td>83.9823</td>
 +
</tr>
 +
</table>
 +
<Br>
 +
<b>WT</b><Br>
 +
<table border>
 +
<tr>
 +
<td>Reaction time </td>
 +
<td> volume </td>
 +
<td> Peak area </td>
 +
<td> Peak area(%) </td>
 +
</tr>
 +
<tr>
 +
<td>10min </td>
 +
<td>20μl</td>
 +
<td>232791.6</td>
 +
<td>83.691</td>
 +
</tr>
 +
</table>　
 +
<Br>
 +
 +
<b>No enzym</b><Br>
 +
<table border>
 +
<tr>
 +
<td>Reaction time </td>
 +
<td> volume </td>
 +
<td> Peak area </td>
 +
<td> Peak area(%) </td>
 +
</tr>
 +
<tr>
 +
<td>10min </td>
 +
<td>20μl</td>
 +
<td>243418.6</td>
 +
<td>84.37</td>
 +
</tr>
 +
</table>　
 +
  
  

Latest revision as of 08:00, 6 October 2012

formate dehydrogenase device

formate dehydrogenase device

Biosafety_Level1.png Safety.png

Constant protein generator of formaldehyde dehydrogenase 4 (FDH4) from Methylobacterium extroquens.
FDH4 is composed of α-subunit and β-subunit. This parts consists both of them.


Decompose quontify

only 2mM formate in medium
Peak = 8.81(min)


BBa_K749015

Reaction time volume Peak area Peak area(%)
30min 50μl 229371.2 80.88
10min 20μl 248294.2 83.9823


WT

Reaction time volume Peak area Peak area(%)
10min 20μl 232791.6 83.691
　


No enzym

Reaction time volume Peak area Peak area(%)
10min 20μl 243418.6 84.37
　


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2297
    Illegal PstI site found at 2009
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2297
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 2009
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2297
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2297
    Illegal PstI site found at 2009
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2297
    Illegal PstI site found at 2009
    Illegal NgoMIV site found at 1245
    Illegal NgoMIV site found at 1508
    Illegal NgoMIV site found at 1696
    Illegal NgoMIV site found at 1736
    Illegal NgoMIV site found at 1866
    Illegal NgoMIV site found at 2246
    Illegal NgoMIV site found at 2497
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 283
    Illegal BsaI site found at 1573
    Illegal BsaI.rc site found at 419
    Illegal BsaI.rc site found at 1112
    Illegal BsaI.rc site found at 1916
    Illegal BsaI.rc site found at 2434
    Illegal BsaI.rc site found at 2611
    Illegal SapI site found at 559
    Illegal SapI.rc site found at 121