Difference between revisions of "Part:BBa K901007"

Latest revision as of 19:09, 2 October 2012

GalU Expression Construct

GalU (BBa_K901004) under control of the LacI promoter (BBa_J04500): galU codes for UDP-Glucose phosphorylase, an E. coli enzyme that produces UDP-glucose.

This expression construct is part of a 3 enzyme system designed to enhance the production and secretion of monosaccharide sugars in Synechococcus elongatus. This system is comprised of InvA (BBa_K901003), Galu, and Glf (BBa_K901008).

GalU codes for UDP-glucose phosphorylase. This is an enzyme from E. coli that produces UDP-glucose. The presence of UDP-glucose enhances the native production of the disaccharide sucrose in S. elongatus in response to salt shock.

Sucrose is then cleaved into glucose and fructose by Invertase A and secreted from the cell via the membrane bound transport protein Glf. These monosaccharides are sufficient nutriment for E. coli and will be used to feed a culture of engineered cells designed to produce high levels of acetic acid.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 272
Illegal NgoMIV site found at 380
Illegal NgoMIV site found at 929
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 707
Illegal BsaI.rc site found at 980

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K901007 short</partinfo>
-pLacI regulated GalU ([https://parts.igem.org/Part:BBa_K901004 BBa_K901004]: an enzyme that cleaves sucrose into glucose and fructose)
+GalU ([https://parts.igem.org/Part:BBa_K901004 BBa_K901004]) under control of the LacI promoter ([https://parts.igem.org/Part:BBa_J04500 BBa_J04500]): galU codes for UDP-Glucose phosphorylase, an <i>E. coli</i> enzyme that produces UDP-glucose.
+This expression construct is part of a 3 enzyme system designed to enhance the production and secretion of monosaccharide sugars in <i>Synechococcus elongatus</i>. This system is comprised of InvA ([https://parts.igem.org/Part:BBa_K901003 BBa_K901003]), Galu, and Glf ([https://parts.igem.org/Part:BBa_K901008 BBa_K901008]).
+GalU codes for UDP-glucose phosphorylase. This is an enzyme from <i>E. coli</i> that produces UDP-glucose. The presence of UDP-glucose enhances the native production of the disaccharide sucrose in <i>S. elongatus</i> in response to salt shock.
+Sucrose is then cleaved into glucose and fructose by Invertase A and secreted from the cell via the membrane bound transport protein Glf. These monosaccharides are sufficient nutriment for <i>E. coli</i> and will be used to feed a culture of engineered cells designed to produce high levels of acetic acid.
 <!-- Add more about the biology of this part here