Difference between revisions of "Part:BBa K921000"
Line 1: Line 1:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K921000 short</partinfo>
 
<partinfo>BBa_K921000 short</partinfo>
 +
<html>
 +
<br>
 +
This is a mutant T7 promoter that includes a lacO sequence. This is repressible by the LacI protein and is induced in the presence of IPTG. The promoter sits in the 5' region of the gene of interest and initiates transcription when the cell expresses T7 RNA polymerase. Be sure you are using a cell strain that is compatible with T7 expression vectors. <br>
 +
The following parameters were calculated and compared against the "wild-type"</html> [https://parts.igem.org/part:BBa_K613007] <html> The following parameters were calculated:
 +
<table border="1">
 +
 +
<tr>
 +
<th>Promoter</th>
 +
<td> <i>BBa K921000</i> </td>
 +
<td> BBa K921001 </td>
 +
<td> BBa K921002 </td>
 +
</tr>
 +
 +
<tr>
 +
<th>Transcription Strength</th>
 +
<td> <i>97%</i> </td>
 +
<td> 72% </td>
 +
<td> 127% </td>
 +
 +
</tr>
 +
 +
<tr>
 +
<th>Translational Efficiency</th>
 +
<td> <i>6%</i></td>
 +
<td> 6%</td>
 +
<td> 94%</td>
 +
 +
</tr>
 +
</table>
 +
</html>
  
−
This is a mutant T7 promoter that includes a lacO sequence. This is repressible by the LacI protein and is induced in the presence of IPTG. The promoter sits in the 5' region of the gene of interest and initiates transcription when the cell expresses T7 RNA polymerase. Be sure you are using a cell strain that includes this gene (DE3).
 
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 01:43, 2 October 2012

T7 RNAP + IPTG->PoPs (Mutant I)
This is a mutant T7 promoter that includes a lacO sequence. This is repressible by the LacI protein and is induced in the presence of IPTG. The promoter sits in the 5' region of the gene of interest and initiates transcription when the cell expresses T7 RNA polymerase. Be sure you are using a cell strain that is compatible with T7 expression vectors.
The following parameters were calculated and compared against the "wild-type" [1] The following parameters were calculated:

Promoter BBa K921000 BBa K921001 BBa K921002
Transcription Strength 97% 72% 127%
Translational Efficiency 6% 6% 94%


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

directionForward
efficiency169%
negative_regulatorsLacI (Hypothesized LacIQ compatible)