Difference between revisions of "Lux"

Revision as of 22:58, 7 June 2006

Lux operon

schematic for the action of the Lux operon

• Basic Information: The lux operon encodes genes for self-regulation and for the production of luminescent proteins. It is originally isolated from the bacterium [http://en.wikipedia.org/wiki/Vibrio_fischeri Vibrio fischeri].
• Component Genes
  • pLuxR: "right hand" lux promoter region
  • LuxR:
  • LuxI:
• Applications: These are based on the "quorum sensing" abilities of the Lux pathway to acyl-homoserine lactones.

more on the Lux operon-based Cell-to-cell signalling Featured Parts...

LuxR

LuxR is a constitutively expressed protein that can bind AHL. When bound to AHL it can stimulate transcription from the right hand lux promoter (pLuxR). In the natural system, this promoter controls transcription of the LuxI enzyme leading to a positive feedback loop that increases transcription from the right hand lux promoter. In addition controlling the transcription of luxI, the promoter also controls transcription of luciferase.

LuxI

LuxI is a synthase that converts SAM into a small molecule called an acyl-homoserine lactone (AHL). It can diffuse across cell membranes and is stable in growth media at a range of pH. In the natural system, the pLuxR promoter controls transcription of the LuxI enzyme leading to a positive feedback loop that increases transcription from the right hand lux promoter. In addition controlling the transcription of luxI, the promoter also controls transcription of luciferase.