Difference between revisions of "Part:BBa K917000:Design"
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===Design Notes=== | ===Design Notes=== | ||
Due to the pathogenic nature of the source strain, purified genomic DNA was obtained from a veterinary laboratory to use as a PCR template, and the E. coli O157:H7 strain was never present in viable form in our laboratory. Note that the native RBS is included. | Due to the pathogenic nature of the source strain, purified genomic DNA was obtained from a veterinary laboratory to use as a PCR template, and the E. coli O157:H7 strain was never present in viable form in our laboratory. Note that the native RBS is included. | ||
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===Source=== | ===Source=== | ||
Latest revision as of 20:37, 1 October 2012
cscA sucrose hydrolase from E. coli 157:H7 Sakai
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 265
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Due to the pathogenic nature of the source strain, purified genomic DNA was obtained from a veterinary laboratory to use as a PCR template, and the E. coli O157:H7 strain was never present in viable form in our laboratory. Note that the native RBS is included.
Source
Escherichia coli O157:H7 strain Sakai genomic DNA
References
Jahreis, K., Bentler, L., Bockmann, J., Hans, S., Meyer, A., Siepelmeyer, J., et al. (2002). Adaptation of sucrose metabolism in the Escherichia coli Wild-Type Strain EC31132. Journal of Bacteriology, 5307-5316