Difference between revisions of "Part:BBa J45006"

Revision as of 20:03, 8 June 2006

alcohol acetyltransferase I (ATF1)

Alcohol acetyltransferase I (ATFI) encodes the enzyme alcohol acetyltransferase I (AATase I), which produces acetate esters from acetyl CoA and alcohols. It was obtained from S. cerevisiae.

Usage and Biology

We will be interested in using AATase I to catalyze the reaction: isoamyl alcohol -> isoamyl acetate. Isoamyl acetate is a volatile ester with a strong banana-like scent. (The related enzyme AATase II, which is encoded by the gene ATF2, performs a similar function...but it is less efficient.)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 99
Illegal BamHI site found at 1426
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 526
Illegal SapI.rc site found at 1534

@@ Line 2: / Line 2: @@
 <partinfo>BBa_J45006 short</partinfo>
-From S. cerevisiae (yeast)
+Alcohol acetyltransferase I (ATFI) encodes the enzyme alcohol acetyltransferase I (AATase I), which produces acetate esters from acetyl CoA and alcohols. It was obtained from ''S. cerevisiae''.
 ===Usage and Biology===
-*The gene ATF1 encodes the enzyme alcohol acetyltransferase I (AATase I), which produces acetate esters from acetyl CoA and alcohols.  We will be interested in using AATase I to catalyze the reaction of isoamyl alcohol -> isoamyl acetate; isoamyl acetate is a volatile ester with a strong banana-like scent. (The related enzyme AATase II, which is encoded by the gene ATF2, performs a similar function...but it is less efficient.)
+We will be interested in using AATase I to catalyze the reaction: isoamyl alcohol -> isoamyl acetate. Isoamyl acetate is a volatile ester with a strong banana-like scent. (The related enzyme AATase II, which is encoded by the gene ATF2, performs a similar function...but it is less efficient.)
-*Horton et al. reported a yield of 1.8mM isoamyl acetate 40 hours after the addition of 10mM isoamyl alcohol to cultured E. coli expressing the ATF1 gene.
 <span class='h3bb'>Sequence and Features</span>