Difference between revisions of "Part:BBa K929106"

Latest revision as of 18:26, 1 October 2012

B-cell transmembrane region with mCherry

General information

B-cell transmembrane region with mCherry
BioBrick Nr.	BBa_929106
RFC standard	RFC 25
Requirement	pSB1C3
Source	Gene synthesis by GeneArt
Submitted by	[http://2012.igem.org/Team:Potsdam_Bioware Potsdam_Bioware2012]

This part is a composite part of the transmembrane domain of a B-cell receptor with a 3 aa linker fused to the mcherry reporter.

Transmembrane domain of B-cell receptor with glycine-serine linker:
Helical single-span transmembrane domain of the B-Cell-Receptor with a flexible 3 aa linker at the C-terminal end. This part was designed for fusion to proteins or peptides that will be presented on the cells surface. A signal peptide at the N-terminal end of the fusion protein for membrane integration (e.g. part BBa_K157001) is highly required.

mCherry:
Expression of the fusion part and its cellular localization can be monitored by the mcherry signal with an excitation at 587 nm and an emission at 610 nm.

Characterization

For characterization and further applications see BBa_K929107.

Usage and Biology

Sequence and Features