Difference between revisions of "Part:BBa K929104"
Line 1: Line 1:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K929104 short</partinfo>
 
<partinfo>BBa_K929104 short</partinfo>
 +
<p style="background-color: rgb(238, 221, 130); font-weight: bold;">General information</p>
 
{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
 
{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
−
! colspan="2" style="background:#66bbff;"|Anti-GFP Nanobody
+
! colspan="2" style="background:rgb(240, 20, 70);"|Anti-GFP Nanobody
 
|-
 
|-
 
! colspan="2"|[[Image:UP12_BBa_K929104.png|300px]]
 
! colspan="2"|[[Image:UP12_BBa_K929104.png|300px]]

Revision as of 18:24, 1 October 2012

Anti-GFP Nanobody

General information

Anti-GFP Nanobody
UP12 BBa K929104.png
BioBrick Nr. BBa_929104
RFC standard RFC 25
Requirement pSB1C3
Source Gene synthesis by GeneArt
Submitted by [http://2012.igem.org/Team:Potsdam_Bioware Potsdam_Bioware2012]


UP12 BBa K929104 vector.png





















The green fluorescent protein (GFP)-nanobody is a single-chain VHH antibody domain developed with specific binding activity against GFP and shows a Kd value of 1.4 nM. Its CDR3 loop is very short and has significantly fewer contacts with the GFP ligand compared to other nanobodies. Furthermore, the shortness of this CDR3 loop leads to the exposure of the framework 2 region, which has a major contribution to the binding with GFP.

Characterization

For characterization and further applications see BBa_K929107.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]