Difference between revisions of "Part:BBa K831017"

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Fluorescence reporter that responds to the presence of 3OH-PAME (Quorum Sensing signal).
 
Fluorescence reporter that responds to the presence of 3OH-PAME (Quorum Sensing signal).
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===Usage and Biology===
 
===Usage and Biology===
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=='''Functional Characterization'''==
 
=='''Functional Characterization'''==
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To assess the fuctionality of our part ''Escherichia coli'' K12 DH5a were transformed with part BBa_K831017
  
 
[[Image:PxpsR-EYFP.png|600px|thumb|center|]]
 
[[Image:PxpsR-EYFP.png|600px|thumb|center|]]
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To assess the fuctionality of our part ''Escherichia coli'' K12 DH5a were transformed with part BBa_K831017
 
  
 
Bacterial strains were photographed while using Differential Interference Contrast (DIC) microscopy and Fluorescence microscopy using FITC filter. Bacteria transformed with PxpsR-eYFP construct show basal levels of expression of the reporter while none of the bacteria transformed with the promotorless EYFP show fluorescence. High background levels in the promotorless EYFP  are due to the autoflorescence of the LB medium and the high exposition time.
 
Bacterial strains were photographed while using Differential Interference Contrast (DIC) microscopy and Fluorescence microscopy using FITC filter. Bacteria transformed with PxpsR-eYFP construct show basal levels of expression of the reporter while none of the bacteria transformed with the promotorless EYFP show fluorescence. High background levels in the promotorless EYFP  are due to the autoflorescence of the LB medium and the high exposition time.
  
 
[[Image:PxpsR-YFP.png|600px|thumb|center|]]
 
[[Image:PxpsR-YFP.png|600px|thumb|center|]]

Revision as of 15:01, 1 October 2012

PxpsR-EYFP

Fluorescence reporter that responds to the presence of 3OH-PAME (Quorum Sensing signal). Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 65
    Illegal NgoMIV site found at 312
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Characterization

To assess the fuctionality of our part Escherichia coli K12 DH5a were transformed with part BBa_K831017

PxpsR-EYFP.png

Bacterial strains were photographed while using Differential Interference Contrast (DIC) microscopy and Fluorescence microscopy using FITC filter. Bacteria transformed with PxpsR-eYFP construct show basal levels of expression of the reporter while none of the bacteria transformed with the promotorless EYFP show fluorescence. High background levels in the promotorless EYFP are due to the autoflorescence of the LB medium and the high exposition time.

PxpsR-YFP.png