Difference between revisions of "Part:BBa K831017"
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=='''Functional Characterization'''== | =='''Functional Characterization'''== | ||
| − | Bacterials strains were photographed while using Differential Interference Contrast (DIC) microscopy and Fluorescence microscopy using FITC filter. Bacteria transformed with PxpsR-eYFP construct show basal levels of expression of the reporter while none of the bacteria transformed with the promotorless | + | Bacterials strains were photographed while using Differential Interference Contrast (DIC) microscopy and Fluorescence microscopy using FITC filter. Bacteria transformed with PxpsR-eYFP construct show basal levels of expression of the reporter while none of the bacteria transformed with the promotorless EYFP show fluorescence. High background levels in the promotorless EYFP are due to the autoflorescence of the LB medium and the high exposition time.]] |
[[Image:PxpsR-YFP.png|550px|thumb|center|'''Image 3'''. '''Functionality of the reporter PxpsR-eYFP''']] | [[Image:PxpsR-YFP.png|550px|thumb|center|'''Image 3'''. '''Functionality of the reporter PxpsR-eYFP''']] | ||
Revision as of 03:26, 1 October 2012
PxpsR-EYFP
Fluorescence reporter that responds to the presence of 3OH-PAME (Quorum Sensing signal)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 65
Illegal NgoMIV site found at 312 - 1000COMPATIBLE WITH RFC[1000]
Functional Characterization
Bacterials strains were photographed while using Differential Interference Contrast (DIC) microscopy and Fluorescence microscopy using FITC filter. Bacteria transformed with PxpsR-eYFP construct show basal levels of expression of the reporter while none of the bacteria transformed with the promotorless EYFP show fluorescence. High background levels in the promotorless EYFP are due to the autoflorescence of the LB medium and the high exposition time.]]
