Difference between revisions of "Part:BBa K831017"

Revision as of 03:24, 1 October 2012

PxpsR-EYFP

Fluorescence reporter that responds to the presence of 3OH-PAME (Quorum Sensing signal)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 65
Illegal NgoMIV site found at 312
1000
COMPATIBLE WITH RFC[1000]

Image 3'. Functionality of the reporter PxpsR-eYFP

@@ Line 19: / Line 19: @@
 =='''Functional Characterization'''==
-The reporter was tested in the pSB1A2 however the part was ensambled into de pSB1C3 backbone and sent to the iGEM Registry Parts. Here we show the basal activity (in absence of 3 OH-PAME) of the PxpsR-eYFP reporter (Image 3) and the functionality
+[[Image:PxpsR-YFP.png|450px|thumb|center|'''Image 3'''. '''Functionality of the reporter PxpsR-eYFP'']]
-[[Image:PxpsR-YFP.png|450px|thumb|center|''Image 3''. '''Functionality of the reporter PxpsR-eYFP'''. Bacterials strains were photographed while using Differential Interference Contrast (DIC) microscopy and Fluorescence microscopy using FITC filter. Bacteria transformed with PxpsR-eYFP construct show basal levels of expression of the reporter while none of the bacteria transformed with the promotorless eYFP show fluorescence. High background levels in the promotorless eYFP  are due to the autoflorescence of the LB medium and the high exposition.]]