Difference between revisions of "Part:BBa K880003"
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This is an asymmetrically digestible reporter, same orientation of inverted repeats as K137058 (external sites located internally of flip region)(IRL - “flip region” - IRR) for assaying fim-Based recombinase activity. | This is an asymmetrically digestible reporter, same orientation of inverted repeats as K137058 (external sites located internally of flip region)(IRL - “flip region” - IRR) for assaying fim-Based recombinase activity. | ||
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| + | <p>https://static.igem.org/mediawiki/2012/e/e6/Asymmetrical_Digest_Reporter_Orientations.png</p> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K880003 SequenceAndFeatures</partinfo> | <partinfo>BBa_K880003 SequenceAndFeatures</partinfo> | ||
Revision as of 00:20, 1 October 2012
A Utility for Assaying fim-Based Recombinases
-IRL K137010 + Sequence with AgeI cut-site K165006 + IRR K137008 Mi8
This is an asymmetrically digestible reporter, same orientation of inverted repeats as K137058 (external sites located internally of flip region)(IRL - “flip region” - IRR) for assaying fim-Based recombinase activity.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 116
- 1000COMPATIBLE WITH RFC[1000]