Difference between revisions of "Part:BBa K741011"

Latest revision as of 07:17, 3 October 2012

plac-anticro-T-pCon(0.244)-RBS-crogfp-T

The fusion protein crogfp(λ phage Cro protein and GFP) is constantly expressed. The antisense RNA of cro is produced to inhibit the translation of the mRNA of crogfp after induced by IPTG.This part is used to measure the function of anticro.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 124
Illegal BglII site found at 1481
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 918

Data

plac-anticro-T-pCon(0.244)-RBS-crogfp-T
OD
t/min	Parallel	0	200mM glucose	400mM glucose	600mM glucose	800mM glucose	1000mM glucose	1000mM IPTG	M9 medium
0	1	0.0973	0.1094	0.1064	0.111	0.107	0.1061	0.0971	0.0442
	2	0.0963	0.107	0.109	0.1053	0.1038	0.1058	0.0968
	3	0.0942	0.1076	0.1084	0.1066	0.1023	0.1068	0.0972
45	1	0.1353	0.1419	0.1534	0.1688	0.1744	0.1768	0.1277
	2	0.1269	0.1414	0.1527	0.1714	0.1918	0.1756	0.1313
	3	0.1286	0.1387	0.1481	0.1682	0.1776	0.1744	0.1248	0.0416
90	1	0.1669	0.1797	0.1841	0.2	0.2051	0.1945	0.18
	2	0.1663	0.1741	0.1836	0.1969	0.1941	0.2088	0.1663
	3	0.1653	0.1761	0.1847	0.1962	0.2023	0.195	0.177	0.0402
135	1	0.2079	0.2085	0.2109	0.2279	0.2272	0.2072	0.2097
	2	0.2144	0.2038	0.2158	0.2104	0.1992	0.2068	0.2052
	3	0.2107	0.2038	0.2031	0.2285	0.225	0.1962	0.2034	0.0411
180	1	0.2214	0.2137	0.2159	0.2331	0.2414	0.2114	0.2132
	2	0.215	0.2048	0.2212	0.2179	0.2081	0.2075	0.2068
	3	0.2132	0.2118	0.2164	0.2325	0.2316	0.1989	0.2074	0.0419
225	1	0.2252	0.2117	0.2093	0.2339	0.2294	0.2082	0.2166
	2	0.2159	0.207	0.2206	0.2173	0.2071	0.2113	0.2082
	3	0.2133	0.2125	0.2178	0.2401	0.2313	0.2009	0.2104	0.0396

plac-anticro-T-pCon(0.244)-RBS-crogfp-T
Fluorescence intensity
t/min	Parallel	0	200mM glucose	400mM glucose	600mM glucose	800mM glucose	1000mM glucose	1000mM IPTG	M9 medium
0	1	81.832	83.434	86.34	85.413	75.242	79.314	80.354	14.031
	2	78.832	83.66	83.362	85.971	80.54	81.981	80.693
	3	83.71	79.357	85.259	80.692	78.863	75.19	77.574
45	1	97.074	96.549	105.152	96.747	109.125	113.189	104.506
	2	105.454	102.764	110.095	106.939	101.639	105.917	102.987
	3	91.59	101.685	112.482	109.825	120.504	102.764	117.71	14.037
90	1	114.849	120.274	128.544	130.12	133.079	136.338	116.663
	2	117.428	124.116	114.105	137.701	134.494	143.047	119.459
	3	115.149	114.123	131.119	128.508	136.205	138.58	119.333	14.013
135	1	149.328	130.73	149.457	138.964	161.753	156.794	136.9
	2	139.522	133.454	145.121	158.924	174.357	157.161	144.882
	3	129.327	141.836	146.587	149.863	156.211	146.249	140.91	14.449
180	1	155.743	167.421	175.027	164.577	190.285	162.935	141.198
	2	166.686	166.272	168.987	177.399	170.245	142.566	148.961
	3	168.156	163.311	172.914	182.473	164.398	145.165	148.663	13.826
225	1	155.82	164.911	176.171	188.184	185.865	163.278	163.715
	2	172.056	160.626	169.286	186.381	169.715	172.464	158.551
	3	171.758	172.442	181.382	192.399	185.049	156.616	159.473	14.123

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K741011 short</partinfo>
-The fusion protein crogfp is constantly expressed and the antisense RNA of cro is produced to inhibit the translation of the mRNA of crogfp after IPTG activated the promoter plac.
+The fusion protein crogfp(λ phage Cro protein and GFP) is constantly expressed. The antisense RNA of cro is produced to inhibit the translation of the mRNA of crogfp after induced by IPTG.This part is used to measure the function of anticro.
 <!-- Add more about the biology of this part here