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Revision as of 17:15, 30 September 2012

plac-anticro-T-pCon(0.244)-RBS-crogfp-T

The fusion protein crogfp is constantly expressed and the antisense RNA of cro is produced to inhibit the translation of the mRNA of crogfp after IPTG activated the promoter plac.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 124
    Illegal BglII site found at 1481
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 918

USTC2012Result9 副本.png

Data

                                     plac-anticro-T-pCon(0.244)-RBS-crogfp-T
                                                    OD
t/min Parallel 0 200mM glucose 400mM glucose 600mM glucose 800mM glucose 1000mM glucose 1000mM IPTG M9 medium
0 1 0.0973 0.1094 0.1064 0.111 0.107 0.1061 0.0971 0.0442
　 2 0.0963 0.107 0.109 0.1053 0.1038 0.1058 0.0968 　
　 3 0.0942 0.1076 0.1084 0.1066 0.1023 0.1068 0.0972 　
45 1 0.1353 0.1419 0.1534 0.1688 0.1744 0.1768 0.1277 　
　 2 0.1269 0.1414 0.1527 0.1714 0.1918 0.1756 0.1313 　
　 3 0.1286 0.1387 0.1481 0.1682 0.1776 0.1744 0.1248 0.0416
90 1 0.1669 0.1797 0.1841 0.2 0.2051 0.1945 0.18 　
　 2 0.1663 0.1741 0.1836 0.1969 0.1941 0.2088 0.1663 　
　 3 0.1653 0.1761 0.1847 0.1962 0.2023 0.195 0.177 0.0402
135 1 0.2079 0.2085 0.2109 0.2279 0.2272 0.2072 0.2097 　
　 2 0.2144 0.2038 0.2158 0.2104 0.1992 0.2068 0.2052 　
　 3 0.2107 0.2038 0.2031 0.2285 0.225 0.1962 0.2034 0.0411
180 1 0.2214 0.2137 0.2159 0.2331 0.2414 0.2114 0.2132 　
　 2 0.215 0.2048 0.2212 0.2179 0.2081 0.2075 0.2068 　
　 3 0.2132 0.2118 0.2164 0.2325 0.2316 0.1989 0.2074 0.0419
225 1 0.2252 0.2117 0.2093 0.2339 0.2294 0.2082 0.2166 　
　 2 0.2159 0.207 0.2206 0.2173 0.2071 0.2113 0.2082 　
　 3 0.2133 0.2125 0.2178 0.2401 0.2313 0.2009 0.2104 0.0396
                                     plac-anticro-T-pCon(0.244)-RBS-crogfp-T
                                               Fluorescence intensity
t/min Parallel 0 200mM glucose 400mM glucose 600mM glucose 800mM glucose 1000mM glucose 1000mM IPTG M9 medium
0 1 81.832 83.434 86.34 85.413 75.242 79.314 80.354 14.031
　 2 78.832 83.66 83.362 85.971 80.54 81.981 80.693 　
　 3 83.71 79.357 85.259 80.692 78.863 75.19 77.574 　
45 1 97.074 96.549 105.152 96.747 109.125 113.189 104.506 　
　 2 105.454 102.764 110.095 106.939 101.639 105.917 102.987 　
　 3 91.59 101.685 112.482 109.825 120.504 102.764 117.71 14.037
90 1 114.849 120.274 128.544 130.12 133.079 136.338 116.663 　
　 2 117.428 124.116 114.105 137.701 134.494 143.047 119.459 　
　 3 115.149 114.123 131.119 128.508 136.205 138.58 119.333 14.013
135 1 149.328 130.73 149.457 138.964 161.753 156.794 136.9 　
　 2 139.522 133.454 145.121 158.924 174.357 157.161 144.882 　
　 3 129.327 141.836 146.587 149.863 156.211 146.249 140.91 14.449
180 1 155.743 167.421 175.027 164.577 190.285 162.935 141.198 　
　 2 166.686 166.272 168.987 177.399 170.245 142.566 148.961 　
　 3 168.156 163.311 172.914 182.473 164.398 145.165 148.663 13.826
225 1 155.82 164.911 176.171 188.184 185.865 163.278 163.715 　
　 2 172.056 160.626 169.286 186.381 169.715 172.464 158.551 　
　 3 171.758 172.442 181.382 192.399 185.049 156.616 159.473 14.123