Difference between revisions of "Part:BBa K812132"
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| − | <b>*** WARNING : THIS PART HAS BEEN MERGED WITH <a href="https://parts.igem.org/wiki/index.php?title=Part: | + | <b>*** WARNING : THIS PART HAS BEEN MERGED WITH <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812032">BBa_K812032</a>. IT IS AVAILABLE IN pSB1C3 AND IN FROG-READY PLASMID BACKBONES <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a>.***</b> |
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Latest revision as of 16:56, 30 September 2012
mCFP with kozak sequence for expression in Xenopus
*** WARNING : THIS PART HAS BEEN MERGED WITH BBa_K812032. IT IS AVAILABLE IN pSB1C3 AND IN FROG-READY PLASMID BACKBONES BBa_K812000.***
mCFP fused with a kozak sequence to enhance its expression in eukaryotic cells. To be used with an eukaryotic expression system such as pSC2+ (K812000) and related parts.
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Tadpole 1 is 2 days (stage 36-38), the expression of CFP is localized in intestine. Tadpole 2 is 4 days (stage 45-46), the expression of CFP is localized in Branchial basket and intestine
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]