Difference between revisions of "Part:BBa K812331"

Revision as of 16:49, 30 September 2012

sfGFP in pCS2+ with HSP70 promoter

*** WARNING : THIS PART HAS BEEN MERGED WITH BBa_K812031. IT IS AVAILABLE IN pSB1C3 AND IN FROG-READY PLASMID BACKBONES BBa_K812000 (CMV promoter), BBa_K812300 (HSP70 promoter), or BBa_K812200 (elastase promoter).***

Super folded Green fluorescent protein (sfGFP) fused with a kozak sequence to enhance its expression in eukaryotic cells. This part is in pCS2+, the plasmid backbone is BBa_K812300
This part was created with the plasmid backbone BBa_K812300: J04450 was replaced by sfGFP (BBa_K812031) and the CMV promoter was replaced by the Hsp70 (Heat Shock Promoter) promoter promoter with SalI and HindIII restriction sites from BBa_K812300.

Sequence and Features