Difference between revisions of "Part:BBa J45001"
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_J45001 parameters</partinfo>
 
<partinfo>BBa_J45001 parameters</partinfo>

Revision as of 21:12, 7 June 2006

SAM:salicylic acid carboxyl methyltransferase; converts salicylic acid to methyl salicylate (winter

From snapdragon (A. Majus)

Usage and Biology

  1. (GenBank Accession No. AF515284)
  2. It is a homodimer with subunit molecular mass of about 45 kDA
  3. On the pET-28a expression vector there is a convenient NdeI site at the initiating ATG codon as well as a BamHI site downstream of the stop codon. Natalia Dudareva is sending the pET-28a vector

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 577
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 901
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 421
    Illegal NgoMIV site found at 425
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 471
    Illegal SapI.rc site found at 75


Functional Parameters

ec_numnone
keggnone
proteinSAMT
swissproQ8H6N2