Difference between revisions of "Part:BBa K845002"
 
Line 6: Line 6:
 
GFP mut2 (S65A V68L S72A): this variant used becomes fluorescent within less than five minutes of transcription initiation, and is highly stable and nonetoxic in <i>E. Coli</i>.
 
GFP mut2 (S65A V68L S72A): this variant used becomes fluorescent within less than five minutes of transcription initiation, and is highly stable and nonetoxic in <i>E. Coli</i>.
  
−
<!-- Add more about the biology of this part here
 
−
===Usage and Biology===
 
  
 +
===Usage and Biology===
 +
Used as a reporter (fluorescence).
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 13:29, 30 September 2012

Strong RBS GFPmut2

RBS shine d'algarno

GFP mut2 (S65A V68L S72A): this variant used becomes fluorescent within less than five minutes of transcription initiation, and is highly stable and nonetoxic in E. Coli.


Usage and Biology

Used as a reporter (fluorescence). Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 670