Difference between revisions of "Part:BBa K779500:Design"
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K779500 short</partinfo> | <partinfo>BBa_K779500 short</partinfo> | ||
Line 7: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
− | + | 4-nucleotide bulge added to BBa_K779501. Bulge placed 10-nt from the 3' end as a potential protection mechanism from cleavage by RISC as suggested by Iba et al. (2011). | |
− | 4-nucleotide bulge added 10-nt from the 3' end as a potential protection mechanism from cleavage by RISC as suggested by Iba et al. (2011). | + | |
See more details at the MIT iGEM wiki: http://2012.igem.org/Team:MIT | See more details at the MIT iGEM wiki: http://2012.igem.org/Team:MIT | ||
− | |||
− | |||
===Source=== | ===Source=== |
Revision as of 20:18, 29 September 2012
Long reporter top strand with bulge MammoBlock
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
4-nucleotide bulge added to BBa_K779501. Bulge placed 10-nt from the 3' end as a potential protection mechanism from cleavage by RISC as suggested by Iba et al. (2011).
See more details at the MIT iGEM wiki: http://2012.igem.org/Team:MIT
Source
De novo design.