Difference between revisions of "Part:BBa K845008"

Revision as of 16:31, 29 September 2012

cyaA with its natural RBS

cyaA gene (from BW25113 E. Coli) encodes the Adenyl cyclase protein which catalyses the conversion of ATP (Adenosine Tri-Phosphate) into cAMP (cyclic Adenosine Mono-Phosphate).

Our saferty note: https://static.igem.org/mediawiki/2012/4/48/CyaA.pdf

Usage and Biology

In Escherichia coli, cAMP is involved in carbon catabolite repression [1] and binds to the cAMP receptor protein (CRP). The corresponding complex (CRP-cAMP) is a transcriptional factor controlling the expression of more than 220 operons [2]. It has been known for a long time that E. coli actively exports cAMP into the growth medium [3][4].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 288
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 922
Illegal SapI.rc site found at 412
Illegal SapI.rc site found at 652

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 Our saferty note: https://static.igem.org/mediawiki/2012/4/48/CyaA.pdf
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 ===Usage and Biology===
+In Escherichia coli, cAMP is involved in carbon catabolite repression [1] and binds to the cAMP receptor protein (CRP). The corresponding complex (CRP-cAMP) is a transcriptional factor controlling the expression of more than 220 operons [2]. It has been known for a long time that E. coli actively exports cAMP into the growth medium [3][4].
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