Difference between revisions of "Part:BBa K845002:Design"
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===Source=== | ===Source=== | ||
| − | The protein comes from Aequorea victoria. This jellyfish uses GFP (Green Fluorescent Protein) in order to convert the blue luminescence emitted by the aequorine into a green luminescence | + | The GFP protein comes from Aequorea victoria. This jellyfish uses GFP (Green Fluorescent Protein) in order to convert the blue luminescence emitted by the aequorine into a green luminescence |
===References=== | ===References=== | ||
Zaslaver, A., Bren, A., Ronen, M., Itzkovitz, S., Kikoin, I., Shavit, S., Liebermeister, W., et al (2006). A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nature Methods, 3(8), 623-628. doi:10.1038/nmeth895 ( http://www.ncbi.nlm.nih.gov/pubmed/16862137 ) | Zaslaver, A., Bren, A., Ronen, M., Itzkovitz, S., Kikoin, I., Shavit, S., Liebermeister, W., et al (2006). A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nature Methods, 3(8), 623-628. doi:10.1038/nmeth895 ( http://www.ncbi.nlm.nih.gov/pubmed/16862137 ) | ||
Revision as of 16:54, 29 September 2012
Strong RBS GFPmut2
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 670
Design Notes
Our safety note: https://static.igem.org/mediawiki/2012/2/2a/ParaBAD-gfp.pdf
Source
The GFP protein comes from Aequorea victoria. This jellyfish uses GFP (Green Fluorescent Protein) in order to convert the blue luminescence emitted by the aequorine into a green luminescence
References
Zaslaver, A., Bren, A., Ronen, M., Itzkovitz, S., Kikoin, I., Shavit, S., Liebermeister, W., et al (2006). A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nature Methods, 3(8), 623-628. doi:10.1038/nmeth895 ( http://www.ncbi.nlm.nih.gov/pubmed/16862137 )