Difference between revisions of "Part:BBa K771001"
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<partinfo>BBa_K771001 short</partinfo> | <partinfo>BBa_K771001 short</partinfo> | ||
− | Membrane anchor 1:RNA aptamer binding protein PP7 [https://parts.igem.org/wiki/index.php?title=Part:BBa_K771111 BBa_K771111]fused with membrane protein | + | Membrane anchor 1:RNA aptamer binding protein PP7 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771111 BBa_K771111]) fused with membrane protein |
− | Lgt[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771102 BBa_K771102]. | + | Lgt([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771102 BBa_K771102]). |
==Overview: Membrane Scaffold System== | ==Overview: Membrane Scaffold System== | ||
[[Image:12SJTU-MA1-MA6.jpg|500px|center]] | [[Image:12SJTU-MA1-MA6.jpg|500px|center]] | ||
− | Demonstration of the whole ''Membrane Scaffold'' device. Membrane Anchor 2, 3, 4, 5 consitutively aggregate while Membrane Anchor 1 and | + | Demonstration of the whole ''Membrane Scaffold'' device. Membrane Anchor 2, 3, 4, 5 consitutively aggregate while Membrane Anchor 1 and 6 only aggregate |
− | with constitutive cluster when certain signal is present. | + | with constitutive cluster (Membrane Anchor 2, 3, 4, 5) when certain signal is present. |
==Backgroud== | ==Backgroud== | ||
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Membrane Anchor 1 and 2 act as a controlling point to determine whether certain pathway (in this case involving enzyme 1, 2, 3, 4 and 5) is activated or | Membrane Anchor 1 and 2 act as a controlling point to determine whether certain pathway (in this case involving enzyme 1, 2, 3, 4 and 5) is activated or | ||
− | not. RNA molecule D0 [https://parts.igem.org/wiki/index.php?title=Part:BBa_K771009 BBa_K771009]with PP7 and MS2 aptamer domain acts as the signal. | + | not. RNA molecule D0([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771009 BBa_K771009]) with PP7 and MS2 aptamer domain acts as the signal. |
[[Image:12SJTU RNASIGNAL.jpg|400px|thumb|center|Sketch of signal RNA molecule (called D0) which consists of PP7 and MS2 aptamer domains. B: Through fusing | [[Image:12SJTU RNASIGNAL.jpg|400px|thumb|center|Sketch of signal RNA molecule (called D0) which consists of PP7 and MS2 aptamer domains. B: Through fusing | ||
− | PP7 and MS2 RNA biding domain [https://parts.igem.org/wiki/index.php?title=Part:BBa_K771111 BBa_K771111] [https://parts.igem.org/wiki/index.php? | + | PP7 and MS2 RNA biding domain ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771111 BBa_K771111] [https://parts.igem.org/wiki/index.php? |
− | title=Part:BBa_K771112 BBa_K771112]to our membrane device, RNA molecule D0[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771009 BBa_K771009] in | + | title=Part:BBa_K771112 BBa_K771112]) to our membrane device, RNA molecule D0[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771009 BBa_K771009] in |
Figure A can function as a bridge to connect different proteins, thus decreasing distance between corresponding enzymes. | Figure A can function as a bridge to connect different proteins, thus decreasing distance between corresponding enzymes. | ||
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==Design== | ==Design== | ||
[[Image:12SJTU_MA1.jpg|400px|thumb|center]] | [[Image:12SJTU_MA1.jpg|400px|thumb|center]] | ||
− | RNA aptamer binding protein PP7 [https://parts.igem.org/wiki/index.php?title=Part:BBa_K771111 BBa_K771111]fused | + | RNA aptamer binding protein PP7([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771111 BBa_K771111]) fused with membrane protein Lgt |
− | + | ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771102 BBa_K771102]). When RNA molecule D0 is present, Membrane Anchor 1 is expected to dimerize with Membrane Anchor 2([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771002 BBa_K771002]) through MS2([https://parts.igem.org/wiki/index.php? | |
− | + | title=Part:BBa_K771112 BBa_K771112]) and PP7([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771111 BBa_K771111]) domain. | |
− | + | ||
− | + | ||
− | + | ||
− | BBa_K771111] domain | + | |
==Related Biobrick== | ==Related Biobrick== | ||
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<br\>Membrabe Anchor 5([[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771005 BBa_K771005]]) | <br\>Membrabe Anchor 5([[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771005 BBa_K771005]]) | ||
<br\>Membrabe Anchor 6([[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771006 BBa_K771006]]) | <br\>Membrabe Anchor 6([[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771006 BBa_K771006]]) | ||
+ | |||
+ | |||
+ | ==Reference== | ||
+ | Delebecque, C. J., A. B. Lindner, et al. (2011). "Organization of intracellular reactions with rationally designed RNA assemblies." Science 333(6041): 470. | ||
+ | |||
==Reference== | ==Reference== |
Revision as of 04:17, 29 September 2012
Membrane Anchor 1 :ssDsbA-Lgt-PP7
Membrane anchor 1:RNA aptamer binding protein PP7 (BBa_K771111) fused with membrane protein
Lgt(BBa_K771102).
Overview: Membrane Scaffold System
Demonstration of the whole Membrane Scaffold device. Membrane Anchor 2, 3, 4, 5 consitutively aggregate while Membrane Anchor 1 and 6 only aggregate
with constitutive cluster (Membrane Anchor 2, 3, 4, 5) when certain signal is present.
Backgroud
Membrane Anchor 1 and 2 act as a controlling point to determine whether certain pathway (in this case involving enzyme 1, 2, 3, 4 and 5) is activated or
not. RNA molecule D0(BBa_K771009) with PP7 and MS2 aptamer domain acts as the signal.
Design
RNA aptamer binding protein PP7(BBa_K771111) fused with membrane protein Lgt
(BBa_K771102). When RNA molecule D0 is present, Membrane Anchor 1 is expected to dimerize with Membrane Anchor 2(BBa_K771002) through MS2([https://parts.igem.org/wiki/index.php?
title=Part:BBa_K771112 BBa_K771112]) and PP7(BBa_K771111) domain.
Related Biobrick
<br\>Membrabe Anchor 2([BBa_K771002]) <br\>Membrabe Anchor 3([BBa_K771003]) <br\>Membrabe Anchor 4([BBa_K771004]) <br\>Membrabe Anchor 5([BBa_K771005]) <br\>Membrabe Anchor 6([BBa_K771006])
Reference
Delebecque, C. J., A. B. Lindner, et al. (2011). "Organization of intracellular reactions with rationally designed RNA assemblies." Science 333(6041): 470.
Reference
Delebecque, C. J., A. B. Lindner, et al. (2011). "Organization of intracellular reactions with rationally designed RNA assemblies." Science 333(6041): 470.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1129
Illegal BglII site found at 1385 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 883
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 504