Difference between revisions of "Part:BBa K771001"

Revision as of 04:12, 29 September 2012

Membrane Anchor 1 :ssDsbA-Lgt-PP7

Membrane anchor 1:RNA aptamer binding protein PP7 BBa_K771111fused with membrane protein

LgtBBa_K771102.

Overview: Membrane Scaffold System

Demonstration of the whole Membrane Scaffold device. Membrane Anchor 2, 3, 4, 5 consitutively aggregate while Membrane Anchor 1 and 6 only aggregate

with constitutive cluster when certain signal is present.

Backgroud

Membrane Anchor 1 and 2 act as a controlling point to determine whether certain pathway （in this case involving enzyme 1, 2, 3, 4 and 5) is activated or

not. RNA molecule D0 BBa_K771009with PP7 and MS2 aptamer domain acts as the signal.

Sketch of signal RNA molecule (called D0) which consists of PP7 and MS2 aptamer domains. B: Through fusing PP7 and MS2 RNA biding domain BBa_K771111 [https://parts.igem.org/wiki/index.php? title=Part:BBa_K771112 BBa_K771112]to our membrane device, RNA molecule D0BBa_K771009 in Figure A can function as a bridge to connect different proteins, thus decreasing distance between corresponding enzymes.

Design

RNA aptamer binding protein PP7 BBa_K771111fused

with membrane protein LgtBBa_K771102. When RNA molecule D0 is present, Membrane Anchor 1 is

expected to dimerize with Membrane Anchor 2BBa_K771002 through MS2

BBa_K771112 and PP7[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771111

BBa_K771111] domain

Related Biobrick

<br\>Membrabe Anchor 2([BBa_K771002]) <br\>Membrabe Anchor 3([BBa_K771003]) <br\>Membrabe Anchor 4([BBa_K771004]) <br\>Membrabe Anchor 5([BBa_K771005]) <br\>Membrabe Anchor 6([BBa_K771006])

Reference

Delebecque, C. J., A. B. Lindner, et al. (2011). "Organization of intracellular reactions with rationally designed RNA assemblies." Science 333(6041): 470.

Sequence and Features