Difference between revisions of "Part:BBa I714891:Experience"

Revision as of 21:34, 14 October 2018

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I714891

measured by fluorescence microscope

http://2012.igem.org/Team:SJTU-BioX-Shanghai 2012 SJTU-BioX-Shanghai team conducted split EGFP assay which could readily help visualize protein interaction. [BBa_K771113] [BBA_K771114]

[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] provided specific characterization for this part by using eGFP for experimental characterization of lentiviral transfer plasmid transfection efficacy and apoptotic effect on colorectal cancer cell line RKO.

User Reviews

UNIQf5a4f6064afb29ab-partinfo-00000001-QINU

BBa_I714891 Tsinghua-D

This part works well for providing eGFP in high expression. We improve this part by adding a sequence of RNAT in from the eGFP gene. Thus, the expression of the reporter gene can be regulated by sensing the temperature.

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UNIQf5a4f6064afb29ab-partinfo-00000003-QINU

@@ Line 12: / Line 12: @@
 [[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771113 BBa_K771113]]
 [[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771114 BBA_K771114]]
+[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] provided specific characterization for this part by using eGFP for experimental characterization of lentiviral transfer plasmid transfection efficacy and apoptotic effect on colorectal cancer cell line RKO.
 ===User Reviews===