Difference between revisions of "Part:BBa K777133"
 
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This part is similar to [https://parts.igem.org/Part:BBa_J61002 J61002] but the backbone is based on pSB1C3 and carries a chloramphenicol resistance.
 
This part is similar to [https://parts.igem.org/Part:BBa_J61002 J61002] but the backbone is based on pSB1C3 and carries a chloramphenicol resistance.
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===Usage and Biology===
 
===Usage and Biology===
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This construct can be useful in different ways, just like the original part [https://parts.igem.org/Part:BBa_J61002 J61002]. However, it is based on pSB1C3 and carries a chloramphenicol resistance.
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# You may use this plasmid to insert a promoter of your choice between the XbaI/EcoRI and SpeI sites. This will provide you with a downstream mRFP as a reporter. Look up the specifications for restriction sites [https://parts.igem.org/Image:PBca1020-r0040.jpg here].
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# Furthermore, you can clone a gene of interest downstream of the constitutive Anderson promoters to express it at a desired rate. For this, the SpeI and PstI sites can be used. Look up the specifications for restriction sites [https://parts.igem.org/Image:PBca1020-r0040.jpg here].
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 14:41, 28 September 2012

Similar to pSB1C3, with RFP reporter

This part is similar to J61002 but the backbone is based on pSB1C3 and carries a chloramphenicol resistance.


Usage and Biology

This construct can be useful in different ways, just like the original part J61002. However, it is based on pSB1C3 and carries a chloramphenicol resistance.

  1. You may use this plasmid to insert a promoter of your choice between the XbaI/EcoRI and SpeI sites. This will provide you with a downstream mRFP as a reporter. Look up the specifications for restriction sites here.
  2. Furthermore, you can clone a gene of interest downstream of the constitutive Anderson promoters to express it at a desired rate. For this, the SpeI and PstI sites can be used. Look up the specifications for restriction sites here.




Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2917
    Illegal XbaI site found at 2932
    Illegal SpeI site found at 1
    Illegal PstI site found at 884
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2917
    Illegal SpeI site found at 1
    Illegal PstI site found at 884
    Illegal NotI site found at 877
    Illegal NotI site found at 2923
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2917
    Illegal XhoI site found at 1901
    Illegal XhoI site found at 2793
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a suffix.
    Illegal SpeI site found at 1
    Illegal PstI site found at 884
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2917
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2932
    Illegal SpeI site found at 1
    Illegal PstI site found at 884
    Illegal AgeI site found at 580
    Illegal AgeI site found at 692
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.



Fig.1: Plasmid map of K777133