Difference between revisions of "Part:BBa K771005"
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<partinfo>BBa_K771005 short</partinfo> | <partinfo>BBa_K771005 short</partinfo> | ||
− | Membrane anchor 5, which consists of interacting light sensor VVD ,PDZ ligand and membrane protein Lgt. Membrane Anchor 5 is proved to constitutively dimerize with Membrane Anchor 4 [[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771004 BBa_K771004]]. and 6 [[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771006 BBa_K771006]]. | + | Membrane anchor 5, which consists of interacting light sensor VVD ,PDZ ligand and membrane protein Lgt. Membrane Anchor 5 is proved to constitutively dimerize with Membrane Anchor 4 [[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771004 BBa_K771004]]. and could only aggregate with Membrane Anchor 6 [[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771006 BBa_K771006]] when blue light is present. |
==Membrane Rudder System== | ==Membrane Rudder System== | ||
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==light sensor== | ==light sensor== | ||
+ | VVD protein is a blue light induced dimer ([[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771109 BBa_K771109]]) | ||
===Related Biobrick=== | ===Related Biobrick=== |
Revision as of 14:01, 28 September 2012
Membrane Anchor 5:ssDsbA-VVD-LGT-GBD Ligand
Membrane anchor 5, which consists of interacting light sensor VVD ,PDZ ligand and membrane protein Lgt. Membrane Anchor 5 is proved to constitutively dimerize with Membrane Anchor 4 [BBa_K771004]. and could only aggregate with Membrane Anchor 6 [BBa_K771006] when blue light is present.
Membrane Rudder System
Demonstration of the whole Membrane Rudder device.
light sensor
VVD protein is a blue light induced dimer ([BBa_K771109])
Related Biobrick
<br\>Membrabe Anchor 1([BBa_K771001]) <br\>Membrabe Anchor 2([BBa_K771002]) <br\>Membrabe Anchor 3([BBa_K771003]) <br\>Membrabe Anchor 4([BBa_K771004]) <br\>Membrabe Anchor 6([BBa_K771006])
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 494
Illegal AgeI site found at 1339 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 960