Difference between revisions of "Part:BBa K771202"

Revision as of 06:14, 28 September 2012

VioB with Membrane Anchor 3

This part is composed with two parts, the Membrane Anchor 2 and Violacein synthetic pathway enzyme vioB. The enzyme can be aggregated with other membrane anchors, composing the Membrane Magic system.For more information, please visit 2012 SJTU-BioX-Shanghai wiki

Usage and Biology

In our Violacein Membrane Rudder System, VioA, VioB, VioE and VioC constitutively aggregate. The violacein biosynthetic pathway includes 5 key enzymes which work in conjunction. VioA, a flavoenzyme and VioB, a heme protein, work together to oxidize and dimerize tryptophen into IPA imine dimer. Then vioE induces a indole rearrangement,producing prodeoxyviolacein acid, also known as PVA.

When blue light is present, VioD with Membrane Anchor 6 would aggregate with complex of VioA,B,C and E, making violacein the dominnant final product.But if you turn the light off, VioD with Membrane Anchor would dissociate with VioA,B,C and E. Thus the biosynthetic pathway for deoxyviolacein is switched on.

VioB with Membrane Anchor 3 (BBa_K771202)

VioE with Membrane Anchor 4 (BBa_K771203)

VioC with Membrane Anchor 5 (BBa_K771204)

VioC with Membrane Anchor 5 (BBa_K771205)

VioD with Membrane Anchor 6 (BBa_K771206)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 3684
Illegal AgeI site found at 904
Illegal AgeI site found at 3880
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 525

@@ Line 4: / Line 4: @@
 This part is composed with two parts, the Membrane Anchor 2 and Violacein synthetic pathway enzyme vioB. The enzyme can be aggregated with other membrane anchors, composing the Membrane Magic system.For more information, please visit 2012 SJTU-BioX-Shanghai wiki
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+In our Violacein Membrane Rudder System, VioA, VioB, VioE and VioC constitutively aggregate. The violacein biosynthetic pathway includes 5 key enzymes which work in conjunction. VioA, a flavoenzyme and VioB, a heme protein, work together to oxidize and dimerize tryptophen into IPA imine dimer. Then vioE induces a indole rearrangement,producing prodeoxyviolacein acid, also known as PVA.
+When blue light is present, VioD with Membrane Anchor 6 would aggregate with complex of VioA,B,C and E, making violacein the dominnant final product.But if you turn the light off, VioD with Membrane Anchor would dissociate with VioA,B,C and E. Thus the biosynthetic pathway for deoxyviolacein is switched on.
+[[Image:12SJTU construction of membrane assembly of violacein.jpg|300px]]
+===Related Parts===
+[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771201 VioA with Membrane Anchor 2 (BBa_K771201)]
+[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771202 VioB with Membrane Anchor 3 (BBa_K771202)]
+[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771203 VioE with Membrane Anchor 4 (BBa_K771203)]
+[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771204 VioC with Membrane Anchor 5 (BBa_K771204)]
+[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771205 VioC with Membrane Anchor 5 (BBa_K771205)]
+[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771206 VioD with Membrane Anchor 6 (BBa_K771206)]
+<!-- Add more about the biology of this part here
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>