Difference between revisions of "Part:BBa K771001"
AleAlejandro (Talk | contribs) (→RNA Rudder) |
AleAlejandro (Talk | contribs) (→Membrane Rudder System) |
||
Line 5: | Line 5: | ||
==Membrane Rudder System== | ==Membrane Rudder System== | ||
[[Image:12SJTU-MA1-MA6.png|500px|center]] | [[Image:12SJTU-MA1-MA6.png|500px|center]] | ||
− | Membrane | + | Demonstration of the whole ''Membrane Rudder'' device. When |
==RNA Rudder== | ==RNA Rudder== |
Revision as of 05:19, 28 September 2012
Membrane Anchor 1 :ssDsbA-Lgt-PP7
Membrane anchor 1:RNA aptamer binding protein PP7 fused with membrane protein Lgt. And it is a part of membrane anchor rudder system.
Membrane Rudder System
Demonstration of the whole Membrane Rudder device. When
RNA Rudder
Membrane Anchor 1 and 2 act as a controlling point to determine whether certain pathway is activated or not.
A: Sketch of signal RNA molecule (called D0) which consists of PP7 and MS2 aptamer domains. B: Through fusing PP7 and MS2 protein to our membrane device, RNA molecule in Figure A can function as a bridge to connect different proteins, thus decreasing distance between corresponding proteins.
Light Rudder
Related Biobrick
<br\>Membrabe Anchor 2([BBa_K771002]) <br\>Membrabe Anchor 3([BBa_K771003]) <br\>Membrabe Anchor 4([BBa_K771004]) <br\>Membrabe Anchor 5([BBa_K771005]) <br\>Membrabe Anchor 6([BBa_K771006])
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1129
Illegal BglII site found at 1385 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 883
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 504