Difference between revisions of "Part:BBa K771001"

(RNA Rudder)
(Membrane Rudder System)
Line 5: Line 5:
 
==Membrane Rudder System==
 
==Membrane Rudder System==
 
[[Image:12SJTU-MA1-MA6.png|500px|center]]
 
[[Image:12SJTU-MA1-MA6.png|500px|center]]
Membrane Anchor 1 and 2 act as a controlling point to determine whether certain pathway is activated or not.
+
Demonstration of the whole ''Membrane Rudder'' device. When
  
 
==RNA Rudder==
 
==RNA Rudder==

Revision as of 05:19, 28 September 2012

Membrane Anchor 1 :ssDsbA-Lgt-PP7

Membrane anchor 1:RNA aptamer binding protein PP7 fused with membrane protein Lgt. And it is a part of membrane anchor rudder system.

Membrane Rudder System

12SJTU-MA1-MA6.png

Demonstration of the whole Membrane Rudder device. When

RNA Rudder

Membrane Anchor 1 and 2 act as a controlling point to determine whether certain pathway is activated or not.

12SJTU RNASIGNAL.jpg

A: Sketch of signal RNA molecule (called D0) which consists of PP7 and MS2 aptamer domains. B: Through fusing PP7 and MS2 protein to our membrane device, RNA molecule in Figure A can function as a bridge to connect different proteins, thus decreasing distance between corresponding proteins.

Light Rudder

Related Biobrick

<br\>Membrabe Anchor 2([BBa_K771002]) <br\>Membrabe Anchor 3([BBa_K771003]) <br\>Membrabe Anchor 4([BBa_K771004]) <br\>Membrabe Anchor 5([BBa_K771005]) <br\>Membrabe Anchor 6([BBa_K771006])

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1129
    Illegal BglII site found at 1385
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 883
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 504