Difference between revisions of "Part:BBa K900001:Design"
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===Source=== | ===Source=== | ||
| − | Synthesized in house | + | Synthesized in house and cloned into addgene plasmid l11911 Previously engineered version of RFP from Patterson and Lippincott-Schwartz, 2002. |
===References=== | ===References=== | ||
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| + | Patterson, G. H. & Lippincott-Schwartz, J. A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells. Science 297, 1873–1877 (2002). | ||
Revision as of 22:31, 3 October 2012
PAGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimization for yeast and internal restriction site removal to be compatible with our Golden Gate cloning scheme.
Source
Synthesized in house and cloned into addgene plasmid l11911 Previously engineered version of RFP from Patterson and Lippincott-Schwartz, 2002.
References
Patterson, G. H. & Lippincott-Schwartz, J. A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells. Science 297, 1873–1877 (2002).