Difference between revisions of "Part:BBa J100099:Experience"
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===Applications of BBa_J100099=== | ===Applications of BBa_J100099=== | ||
We pipetted 200 microliters of one solution containing E coli cells from a small colony and the activators, one with cells from a small colony and no activators, one containing cells from a large colony and the activators, and one containing cells from a large colony and no activators. We also did a positive control with E coli cells containing a known promoter that causes red florescence (J04450) and a negative control with cells containing a the transcriptional terminator that does not cause red fluorescence (J100091). We tested both fluorescence of our samples using a fluorometer and the light absorbance using a spectrophotometer. We measured the fluorescence and absorbance of five samples of each solution, including a control solution that just contained the growth medium. We averaged the values for each solution and subtracted the average fluorescence/absorbance of the control. We then divided the average fluorescence by the average absorbance for each solution. These values are displayed on the accompanying graph. | We pipetted 200 microliters of one solution containing E coli cells from a small colony and the activators, one with cells from a small colony and no activators, one containing cells from a large colony and the activators, and one containing cells from a large colony and no activators. We also did a positive control with E coli cells containing a known promoter that causes red florescence (J04450) and a negative control with cells containing a the transcriptional terminator that does not cause red fluorescence (J100091). We tested both fluorescence of our samples using a fluorometer and the light absorbance using a spectrophotometer. We measured the fluorescence and absorbance of five samples of each solution, including a control solution that just contained the growth medium. We averaged the values for each solution and subtracted the average fluorescence/absorbance of the control. We then divided the average fluorescence by the average absorbance for each solution. These values are displayed on the accompanying graph. | ||
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[[Image:graphs1.png]] | [[Image:graphs1.png]] | ||
Latest revision as of 19:55, 27 September 2012
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Applications of BBa_J100099
We pipetted 200 microliters of one solution containing E coli cells from a small colony and the activators, one with cells from a small colony and no activators, one containing cells from a large colony and the activators, and one containing cells from a large colony and no activators. We also did a positive control with E coli cells containing a known promoter that causes red florescence (J04450) and a negative control with cells containing a the transcriptional terminator that does not cause red fluorescence (J100091). We tested both fluorescence of our samples using a fluorometer and the light absorbance using a spectrophotometer. We measured the fluorescence and absorbance of five samples of each solution, including a control solution that just contained the growth medium. We averaged the values for each solution and subtracted the average fluorescence/absorbance of the control. We then divided the average fluorescence by the average absorbance for each solution. These values are displayed on the accompanying graph.
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