Difference between revisions of "Part:BBa K864006"
| Line 7: | Line 7: | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | This part is available as pSB4A15-[[Part: | + | This part is available as pSB4A15-[[Part:BBa_K864120|pUCori-red]]. This facilitates high plasmid yields and fast red color expression on transformation plates. |
<!-- --> | <!-- --> | ||
Latest revision as of 19:19, 27 September 2012
Low copy BioBrick standard vector
pSB4K15(FRT) is a BioBrick standard vector with low copy pSC101 replication origin BBa_K864050 (~5 copies per cell) and kanamycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
The resistance cassette is flanked by FRT sites (BBa_J61020) which allows for subsequent removal of the resistance cassette from the chromosome by the FLP recombinase.
Usage and Biology
This part is available as pSB4A15-pUCori-red. This facilitates high plasmid yields and fast red color expression on transformation plates.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3711
Illegal NheI site found at 2425
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 3717 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3711 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 3711
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 3711
Plasmid lacks a suffix.
Illegal XbaI site found at 3726
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 2319 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.