Difference between revisions of "Part:BBa K953000"
Line 2: Line 2:
 
<partinfo>BBa_K953000 short</partinfo>
 
<partinfo>BBa_K953000 short</partinfo>
  
−
<i>Synechocystis</i> Heme Oxygenase with a RBS and codon optimised for expression in <i>E. coli</i>. This part differs from <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K953004">Part BBa_K953004</a></html> in that it also includes a T7 promoter (<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">Part BBa_I712074</a></html>). Heme oxygenase will oxidise heme into biliverdin and can then  complex with bacteriophytochrome(<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K953001">Part BBa_K953001</a></html> or <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K953002">Part BBa_K953002</a></html>). This complex can then absorb red light (620-750 nm) to excite the bacteriophytochrome and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time. As E. coli does not produce biliverdin, heme oxygenase  (<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K953004">Part BBa_K953004</a></html>) can be coupled with this part to activate the oxidation of heme to produce biliverdin.
+
<i>Synechocystis</i> Heme Oxygenase with a RBS and codon optimised for expression in <i>E. coli</i>. This part differs from <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K953004">Part BBa_K953004</a></html> in that it also includes a T7 promoter (<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">Part BBa_I712074</a></html>). Heme oxygenase will oxidise heme into biliverdin and can then  complex with bacteriophytochrome (<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K953001">Part BBa_K953001</a></html> or <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K953002">Part BBa_K953002</a></html>). This complex can then absorb red light (620-750 nm) to excite the bacteriophytochrome and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time. As E. coli does not produce biliverdin, heme oxygenase  (<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K953004">Part BBa_K953004</a></html>) can be coupled with this part to activate the oxidation of heme to produce biliverdin.
  
  

Revision as of 00:49, 27 September 2012

Heme Oxygenase with T7 & RBS (E. coli Codon Optimised)

Synechocystis Heme Oxygenase with a RBS and codon optimised for expression in E. coli. This part differs from Part BBa_K953004 in that it also includes a T7 promoter (Part BBa_I712074). Heme oxygenase will oxidise heme into biliverdin and can then complex with bacteriophytochrome (Part BBa_K953001 or Part BBa_K953002). This complex can then absorb red light (620-750 nm) to excite the bacteriophytochrome and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time. As E. coli does not produce biliverdin, heme oxygenase (Part BBa_K953004) can be coupled with this part to activate the oxidation of heme to produce biliverdin.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 314
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]