Difference between revisions of "Part:BBa K953000"

Revision as of 00:49, 27 September 2012

Heme Oxygenase with T7 & RBS (E. coli Codon Optimised)

Synechocystis Heme Oxygenase with a RBS and codon optimised for expression in E. coli. This part differs from Part BBa_K953004 in that it also includes a T7 promoter (Part BBa_I712074). Heme oxygenase will oxidise heme into biliverdin and can then complex with bacteriophytochrome(Part BBa_K953001 or (Part BBa_K953002. This complex can then absorb red light (620-750 nm) to excite the bacteriophytochrome and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time. As E. coli does not produce biliverdin, heme oxygenase (Part BBa_K953004) can be coupled with this part to activate the oxidation of heme to produce biliverdin.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 314
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K953000 short</partinfo>
-<i>Synechocystis</i> Heme Oxygenase with a RBS and codon optimised for expression in <i>E. coli</i>. This part differs from <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K953004">Part BBa_K953004</a></html> in that it also includes a T7 promoter (<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">Part BBa_I712074</a></html>). , oxidises Heme into biliverdin which absorbs light in the 360 nm and 650 nm wavelength producing a green colouration.
+<i>Synechocystis</i> Heme Oxygenase with a RBS and codon optimised for expression in <i>E. coli</i>. This part differs from <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K953004">Part BBa_K953004</a></html> in that it also includes a T7 promoter (<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">Part BBa_I712074</a></html>). Heme oxygenase will oxidise heme into biliverdin and can then  complex with bacteriophytochrome(<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K953001">Part BBa_K953001</a></html> or (<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K953002">Part BBa_K953002</a></html>. This complex can then absorb red light (620-750 nm) to excite the bacteriophytochrome and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time. As E. coli does not produce biliverdin, heme oxygenase  (<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K953004">Part BBa_K953004</a></html>) can be coupled with this part to activate the oxidation of heme to produce biliverdin.
 <!-- Add more about the biology of this part here