Difference between revisions of "Part:BBa K812130"

Latest revision as of 16:56, 30 September 2012

Citrine reporter with a Kozak sequence for expression in Xenopus

*** WARNING : THIS PART HAS BEEN MERGED WITH BBa_K812030. IT IS AVAILABLE IN pSB1C3 AND IN FROG-READY PLASMID BACKBONES BBa_K812000.***

Citrine gene flanked by a kozak sequence for eukaryotic expression in biobrick format. This is designed to be used in mammalian and ovarion expression systems. The backbone of the plasmid is pCS2+ with the CMV promoter is BBa_K812000 and the J04450 is replaced by the mCitrine BBa_K812030, it is ready for immediate use in Xenopus.
This part was characterized and the result is below. Tadpoles have 4 days (near stage 46) and the expression of mCitrine is localized in tail's muscles. perdu
Sequence and Features BBa_K812130 SequenceAndFeatures

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 <partinfo>BBa_K812130 short</partinfo>
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+<b>*** WARNING : THIS PART HAS BEEN MERGED WITH <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812030">BBa_K812030</a>. IT IS AVAILABLE IN pSB1C3 AND IN FROG-READY PLASMID BACKBONES <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a>.***</b>
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 Citrine gene flanked by a kozak sequence for eukaryotic expression in biobrick format. This is designed to be used in mammalian and ovarion expression systems. The backbone of the plasmid is pCS2+ with the CMV promoter is <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a> and the J04450 is replaced by the mCitrine <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812030">BBa_K812030</a>, it is ready for immediate use in Xenopus.
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 Tadpoles have 4 days (near stage 46) and the expression of mCitrine is localized in tail's muscles.
 <img src="https://static.igem.org/mediawiki/2012/b/b6/Picture_citrine_7.09.jpg" alt="perdu" width="500px" /><br/>
-</html>