Difference between revisions of "Part:BBa K812030"

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</br>This part was characterized in pCS2+ <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a> which contains the CMV promoter. </br> J04450 was replaced by this part, it built the BioBrick <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812130">BBa_K812130</a>. This plasmid (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812133">BBa_K812133</a>) express mCitrine into <i>Xenopus</i>: <br/>
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</br>This part was characterized in pCS2+ <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a> which contains the CMV promoter. </br> J04450 was replaced by this part, it built the BioBrick <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812130">BBa_K812130</a>. The plasmid (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K81213">BBa_K81213</a>) which contains this part express mCitrine into <i>Xenopus</i>: <br/>
  
 
Tadpoles have 4 days (near stage 46) and the expression of mCitrine is localized in tail's muscles.
 
Tadpoles have 4 days (near stage 46) and the expression of mCitrine is localized in tail's muscles.

Revision as of 23:56, 26 September 2012

mCitrine reporter with a Kozak sequence for eukaryotic expression

mCitrine gene flanked by a kozak sequence for eukaryotic expression in biobrick format. This part is in pSB1C3.


This part was characterized in pCS2+ BBa_K812000 which contains the CMV promoter.
J04450 was replaced by this part, it built the BioBrick BBa_K812130. The plasmid (BBa_K81213) which contains this part express mCitrine into Xenopus:
Tadpoles have 4 days (near stage 46) and the expression of mCitrine is localized in tail's muscles. perdu

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 667
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]