Difference between revisions of "Part:BBa K801077"
Line 2: Line 2:
 
<partinfo>BBa_K801077 short</partinfo>
 
<partinfo>BBa_K801077 short</partinfo>
  
−
This BioBrick is the final "caffeine synthesis device". It contains all three necessary enzymes:  CaXMT1, CaMXMT1 and CaDXMT1, i.e. it is made up of the three single expression cassettes for each enzyme (see also [https://parts.igem.org/Part:BBa_K801073 BBa_K801073], [https://parts.igem.org/Part:BBa_K801074 BBa_K801074 and [https://parts.igem.org/Part:BBa_K801075 BBa_K801075]). It can be directly transformed into competent yeast cells or cloned in an adequate yeast genome integration vector.  
+
This BioBrick is the final "caffeine synthesis device". It contains all three necessary enzymes:  CaXMT1, CaMXMT1 and CaDXMT1, i.e. it is made up of the three single expression cassettes for each enzyme (see also [https://parts.igem.org/Part:BBa_K801073 BBa_K801073], [https://parts.igem.org/Part:BBa_K801074 BBa_K801074] and [https://parts.igem.org/Part:BBa_K801075 BBa_K801075]). It can be directly transformed into competent yeast cells or cloned in an adequate yeast genome integration vector.  
  
  

Revision as of 22:58, 26 September 2012

Caffeine Synthesis Pathway pTEF2-XMT1-tADH1-pTEF1-MXMT1-tADH1-pTEF2-DXMT1-tADH1

This BioBrick is the final "caffeine synthesis device". It contains all three necessary enzymes: CaXMT1, CaMXMT1 and CaDXMT1, i.e. it is made up of the three single expression cassettes for each enzyme (see also BBa_K801073, BBa_K801074 and BBa_K801075). It can be directly transformed into competent yeast cells or cloned in an adequate yeast genome integration vector.


The expression of the enzymes CaXMT1 (Coffea arabica xanthosine-N-methyl-transferase 1, BBa_K801070) and CaDXMT1 (Coffea arabica dimethyl-xanthine N-methyl transferase 1, = caffeine synthase, BBa_K801072) is controlled by the TEF2-promoter (BBa_K801010) and the ADH1-terminator (BBa_K801012)


The expression of CaMXMT1 (Coffea arabica methyl-xanthine N-methyl transferase 1, BBa_K801071) is controlled by the TEF1 promoter (BBa_K319003) and the ADH1-terminator (BBa_K801012)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3613
    Illegal BglII site found at 5829
    Illegal BglII site found at 5925
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 138
    Illegal NgoMIV site found at 4317
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2385