Difference between revisions of "Part:BBa K801095:Design"
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'''Related BioBrick:'''
 
'''Related BioBrick:'''
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<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
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Other versions: [https://parts.igem.org/Part:BBa_K801094 BBa_K801094: CHS + yeast consensus sequence]
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Related BioBricks: [https://parts.igem.org/Part:BBa_K801090 BBa_K801090], [https://parts.igem.org/Part:BBa_K801091 BBa_K801091], [https://parts.igem.org/Part:BBa_K801092 BBa_K801092], [https://parts.igem.org/Part:BBa_K801093 BBa_K801093], [https://parts.igem.org/Part:BBa_K801094 BBa_K801094], [https://parts.igem.org/Part:BBa_K801096 BBa_K801096], [https://parts.igem.org/Part:BBa_K801097 BBa_K801097], [https://parts.igem.org/Part:BBa_K801098 BBa_K801098]  
  
 
'''Cloning details:'''<br>
 
'''Cloning details:'''<br>
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Designed in RFC25
 
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Revision as of 09:18, 3 October 2012

Naringenin - chalcone synthase (CHS) coding region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 717
    Illegal BsaI.rc site found at 1138
    Illegal SapI.rc site found at 840


Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA

http://www.ncbi.nlm.nih.gov/nucleotide/11096318/

PMID: 15932991


Keywords: Coumaryol-CoA, Coumaroyl-Coenzyme A, Xanthohumol, Biosynthesis, 4-coumarate-coenzyme A ligase, aromatic prenyltransferase


Abbreviations: CHS = naringenin-chalcone synthase

Design Notes

Related BioBrick:

Other versions: BBa_K801094: CHS + yeast consensus sequence

Related BioBricks: BBa_K801090, BBa_K801091, BBa_K801092, BBa_K801093, BBa_K801094, BBa_K801096, BBa_K801097, BBa_K801098

Cloning details:
Designed in RFC25

Quality control measures:
Part was totally sequenced

Backbone:
Backbone name: pSB1C3 Resistance: Cp

Protein coding:
Protein: naringenin-chalcone synthase

Enzymatic activity:
EC-number: 2.3.1.74

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:
Plasmid: pKS2µHyg-PAL-4CL-CHS, provided by John A. Morgan, Purdue University, USA


Organism:
Genesequence derived from Hypericum androsaemum

References

Literature references:
[http://www.ncbi.nlm.nih.gov/pubmed/15932991 Pubmed : Jiang, H., Wood, K.V., Morgan, J.A., 2005. Metabolic Engineering of the Phenylpropanoid Pathway in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 71, 2962–2969]


Database references:
[http://www.ncbi.nlm.nih.gov/nuccore/AF315345.1 GenBank: Hypericum androsaemum chalcone synthase mRNA, complete cds]

[http://www.uniprot.org/uniprot/Q9FUB7 Uniprot: Chalcone synthase]