Difference between revisions of "Part:BBa K812233"
Line 5: Line 5:
 
</br>
 
</br>
 
sfGFP is expressed under the control of the pancreas tissue specific promoter elastase. This part contains the Backbone Biobrick (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812200">BBa_K812200</a>) and the part   
 
sfGFP is expressed under the control of the pancreas tissue specific promoter elastase. This part contains the Backbone Biobrick (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812200">BBa_K812200</a>) and the part   
−
(<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812031">BBa_K812031</a>) instead of J04450.</br>
+
(<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812031">BBa_K812031</a>) instead of J04450.</br></br>
  
−
Elastase is a tissue specific promoter for the pancreas. This backbone is characterized using sfGFP as reporter.</br>The result below is not strong enough and need more information to be sure that the plasmid works well (only one tadpole produced sfGFP into the pancreas).
+
Elastase is a tissue specific promoter for the pancreas. This backbone is characterized using sfGFP as reporter.</br></br>The result below is not strong enough and need more information to be sure that the plasmid works well (only one tadpole produced sfGFP into the pancreas).
  
 
Tadpole at stage 47 produced sfGFP in pancreas, you can compare the auto-fluorescence with the control and the picture with RFP filter compare to GFP.</br>
 
Tadpole at stage 47 produced sfGFP in pancreas, you can compare the auto-fluorescence with the control and the picture with RFP filter compare to GFP.</br>

Revision as of 22:39, 26 September 2012

sfGFP in pCS2+ with elastase promoter


sfGFP is expressed under the control of the pancreas tissue specific promoter elastase. This part contains the Backbone Biobrick (BBa_K812200) and the part (BBa_K812031) instead of J04450.

Elastase is a tissue specific promoter for the pancreas. This backbone is characterized using sfGFP as reporter.

The result below is not strong enough and need more information to be sure that the plasmid works well (only one tadpole produced sfGFP into the pancreas). Tadpole at stage 47 produced sfGFP in pancreas, you can compare the auto-fluorescence with the control and the picture with RFP filter compare to GFP.
perdu

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 19