Difference between revisions of "Part:BBa K812331"

Revision as of 16:46, 30 September 2012

sfGFP in pCS2+ with HSP70 promoter

*** WARNING : THIS PART HAS BEEN MERGED WITH BBa_K812031. IT IS AVAILABLE IN pSB1C3 AND IN FROG-READY PLASMID BACKBONES BBa_K812300.***

Super folded Green fluorescent protein (sfGFP) fused with a kozak sequence to enhance its expression in eukaryotic cells. This part is in pCS2+, the plasmid backbone is BBa_K812300
This part was created with the plasmid backbone BBa_K812300: J04450 was replaced by sfGFP (BBa_K812031) and the CMV promoter was replaced by the Hsp70 (Heat Shock Promoter) promoter promoter with SalI and HindIII restriction sites from BBa_K812300.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 19

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 __NOTOC__
 <partinfo>BBa_K812331 short</partinfo>
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 </br>
-Super folded GFP fused with a kozak sequence to enhance its expression in eukaryotic cells. This part is in pCS2+, the plasmid backbone is <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a>
+<b>*** WARNING : THIS PART HAS BEEN MERGED WITH <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812031">BBa_K812031</a>. IT IS AVAILABLE IN pSB1C3 AND IN FROG-READY PLASMID BACKBONES <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812300">BBa_K812300</a>.***</b>
+</br></br>
+Super folded Green fluorescent protein (sfGFP) fused with a kozak sequence to enhance its expression in eukaryotic cells. This part is in pCS2+, the plasmid backbone is <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812300">BBa_K812300</a>
-</br>This part was  created with the plasmid backbone <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a>: J04450 was replaced by sfGFP (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812031">BBa_K812031</a>) and the CMV promoter was replaced by the Hsp70 promoter promoter with SalI and HindIII restriction sites from <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812031">BBa_K812300</a>.
+</br>This part was  created with the plasmid backbone <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812300">BBa_K812300</a>: J04450 was replaced by sfGFP (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812031">BBa_K812031</a>) and the CMV promoter was replaced by the Hsp70 (Heat Shock Promoter) promoter promoter with SalI and HindIII restriction sites from <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812031">BBa_K812300</a>.