Difference between revisions of "Part:BBa K801011:Design"
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===Source=== | ===Source=== | ||
− | '''Source:'''<br> | + | '''Source:'''<br> PCR product from genomic DNA of ''Saccharomyces cerevisiae'' |
<!--*Commercial system: plasmid name, system name, company name--> | <!--*Commercial system: plasmid name, system name, company name--> | ||
<!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?--> | <!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?--> | ||
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<!--'''Reverse Primer:'''<br><code>5'- ??? - 3'</code><br>--> | <!--'''Reverse Primer:'''<br><code>5'- ??? - 3'</code><br>--> | ||
− | '''Organism:'''<br> | + | '''Organism:'''<br> ''Saccharomyces cerevisiae'' |
<!--*Genesequence derived from ''?organism_name?''--> | <!--*Genesequence derived from ''?organism_name?''--> | ||
<!--*Codonoptimized for ''?organism_name?''--> | <!--*Codonoptimized for ''?organism_name?''--> | ||
<!--*Designed for the following Chassis: ''?organism-name?''--> | <!--*Designed for the following Chassis: ''?organism-name?''--> | ||
<!--*Statement about functionality in other chassis.--> | <!--*Statement about functionality in other chassis.--> | ||
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===References=== | ===References=== |
Revision as of 22:07, 26 September 2012
TEF1 yeast terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Note: The submitted version of this part has a mutation in the BioBrick suffix: The NdeI restriction site has been lost. However, the part can still be used, because the SpeI and PstI restriction sites are functional.
Extracted from genomic DNA of S. cerevisiae using PCR.
Keywords:
Abbreviations:
Design Notes
Related BioBrick:
Quality control measures:
Test digestion using XbaI+PstI-HF, EcorI+ SpeI-HF
- Sequencing using primer VR and VF2
Backbone:
Backbone name: pSB1C3
Protein coding:
Enzymatic activity:
Cytotoxicity:
none
Safety notes:
none
Intellectual property:
Corresponding part author/authors:
Source
Source:
PCR product from genomic DNA of Saccharomyces cerevisiae
Organism:
Saccharomyces cerevisiae
References
Literature references:
Database references: