Difference between revisions of "Part:BBa K895007:Experience"

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===Applications of BBa_K895007===
 
===Applications of BBa_K895007===
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===Results===
 
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<i>E. coli</i> MG1655 was transformed with <partinfo>BBa_K895007</partinfo> grown aerobically overnight in LB medium. The culture was then used to inoculate 30 ml anaerobic cultures in LB only, LB + 0.4% (w/v) tetrathionate or LB + 0.4% (w/v) thiosulfate. Following 16 hours growth aliquotes were taken and analysed by SDS-PAGE and Western immunoblotting using an anti-GFP monoclonal.
 
  
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This small scale aassay reveals that GFP was only expressed from the plasmid in the presence of tetrathionate. The device therefore works as a putative <b>inflammation biosensor</b>.
 
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[[Image:Dundee_Biosensor_Figure5.jpg]]
 
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:Figure 1: Production of GFP from <partinfo>BBa_K895007</partinfo> is tetrathionate dependent. The RED ARROW points to the lane producing GFP.
 
  
 
===User Reviews===
 
===User Reviews===

Revision as of 08:48, 29 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K895007

User Reviews

UNIQ8a71473e7bfa1a1d-partinfo-00000000-QINU UNIQ8a71473e7bfa1a1d-partinfo-00000001-QINU