Difference between revisions of "Part:BBa K895007:Experience"

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===Results===
 
===Results===
 
<i>E. coli</i> MG1655 was transformed with <partinfo>BBa_K895007</partinfo> grown aerobically overnight in LB medium. The culture was then used to inoculate 30 ml anaerobic cultures in LB only, LB + 0.4% (w/v) tetrathionate or LB + 0.4% (w/v) thiosulfate. Following 16 hours growth aliquotes were taken and analysed by SDS-PAGE and Western immunoblotting using an anti-GFP monoclonal.
 
<i>E. coli</i> MG1655 was transformed with <partinfo>BBa_K895007</partinfo> grown aerobically overnight in LB medium. The culture was then used to inoculate 30 ml anaerobic cultures in LB only, LB + 0.4% (w/v) tetrathionate or LB + 0.4% (w/v) thiosulfate. Following 16 hours growth aliquotes were taken and analysed by SDS-PAGE and Western immunoblotting using an anti-GFP monoclonal.
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[[Image:Dundee_Biosensor_Figure5.jpg]]
 
[[Image:Dundee_Biosensor_Figure5.jpg]]
 
:Figure 1: Production of GFP from <partinfo>BBa_K895007</partinfo> is tetrathionate dependent. The RED ARROW points to the lane producing GFP.
 
:Figure 1: Production of GFP from <partinfo>BBa_K895007</partinfo> is tetrathionate dependent. The RED ARROW points to the lane producing GFP.
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Revision as of 21:37, 26 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

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UNIQ2d92dafd5f71c333-partinfo-00000000-QINU UNIQ2d92dafd5f71c333-partinfo-00000001-QINU