Difference between revisions of "Part:BBa K801001"
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This part corresponds to pTUM100 (BBa_K801000). The constitutive pTEF1 promoter has been cloned into the MCS (RFC25 prefix and suffix).
 
This part corresponds to pTUM100 (BBa_K801000). The constitutive pTEF1 promoter has been cloned into the MCS (RFC25 prefix and suffix).
  
 +
'''Vector is compatible with RFC10 and 25'''
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 21:01, 26 September 2012

pTUM101 yeast shuttle vector with pTEF1 promoter

This part corresponds to pTUM100 (BBa_K801000). The constitutive pTEF1 promoter has been cloned into the MCS (RFC25 prefix and suffix).

Vector is compatible with RFC10 and 25 Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 36
    Illegal suffix found at 506
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 36
    Illegal NheI site found at 3771
    Illegal SpeI site found at 507
    Illegal PstI site found at 521
    Illegal NotI site found at 42
    Illegal NotI site found at 514
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 36
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 36
    Illegal suffix found at 507
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 36
    Plasmid lacks a suffix.
    Illegal XbaI site found at 51
    Illegal SpeI site found at 507
    Illegal PstI site found at 521
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 261
    Illegal BsaI.rc site found at 1928
    Illegal BsaI.rc site found at 2874
    Illegal SapI site found at 845
    Illegal SapI site found at 3026