Difference between revisions of "Part:BBa K801076:Design"

(→‎Design Notes)
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'''Cytotoxicity:'''<br>
 
'''Cytotoxicity:'''<br>
−
This Biobrick is one part of the pathway, which leads to caffeine (the BioBrick BBa_K801075 is further needed). We investigated the toxicity of caffeine and found out, that the growth of yeast strains was inhibited at concentrations of 10 mM. Caffeine with a concentration of 100 mM even showed a lethal effect on yeast cells. Lower concentrations of caffeine (range of mikro molar) did not show any growth inhibition or effect.
+
This Biobrick is one part of the pathway, which leads to caffeine (the BioBrick BBa_K801075 is further needed). We investigated the toxicity of caffeine and found out, that the growth of yeast strains was inhibited at concentrations of 10 mM. Caffeine with a concentration of 100 mM even showed a lethal effect on yeast cells. Lower concentrations of caffeine (range of micro molar) did not show any growth inhibition or effect.
 
<!--none/not known/cytotoxic for ''organism name''-->
 
<!--none/not known/cytotoxic for ''organism name''-->
  

Revision as of 19:30, 26 September 2012

Yeast expression cassette for CaXMT1 and CaMXMT1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3613
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 138
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2385

Composite part of BBa_K801010, BBa_K801070, BBa_K801012, BBa_K319003, BBa_K801071 and BBa_K801012


Keywords:


Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:

Backbone:

Protein coding:

Enzymatic activity:

Cytotoxicity:
This Biobrick is one part of the pathway, which leads to caffeine (the BioBrick BBa_K801075 is further needed). We investigated the toxicity of caffeine and found out, that the growth of yeast strains was inhibited at concentrations of 10 mM. Caffeine with a concentration of 100 mM even showed a lethal effect on yeast cells. Lower concentrations of caffeine (range of micro molar) did not show any growth inhibition or effect.

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:


Organism:


References

Literature references:

Database references: